Nanoemulsion and Nanogel Containing <i>Eucalyptus globulus</i> Essential Oil; Larvicidal Activity and Antibacterial Properties

Alipanah, Hiva; Abdollahi, Abbas; Firooziyan, Samira; Zarenezhad, Elham; Jafari, Mojtaba; Osanloo, Mahmoud

doi:https://doi.org/10.1155/2022/1616149

Interdisciplinary Perspectives on Infectious Diseases

On this page

Abstract Background Methods Results Discussion Conclusions Abbreviations Data Availability Ethical Approval Conflicts of Interest Authors’ Contributions Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2022 | Article ID 1616149 | https://doi.org/10.1155/2022/1616149

Nanoemulsion and Nanogel Containing Eucalyptus globulus Essential Oil; Larvicidal Activity and Antibacterial Properties

Hiva Alipanah,¹Abbas Abdollahi,²Samira Firooziyan,³Elham Zarenezhad,⁴Mojtaba Jafari,⁵and Mahmoud Osanloo⁶

Academic Editor: Sadegh Ghorbani-Dalini

Received22 Jun 2022

Revised28 Jul 2022

Accepted18 Aug 2022

Published31 Aug 2022

Abstract

Eucalyptus globulus essential oil (EGEO) possesses many biological effects such as antibacterial, antifungal, and insecticide properties. In the current study, the chemical composition of EGEO was first investigated using GC-MS analysis. Then, a nanoemulsion and nanogel containing EGEO (EGEO-nanoemulsion and EGEO-nanogel) were prepared. After that, the successful loading of EGEO was confirmed using ATR-FTIR analysis. EGEO-nanoemulsion and EGEO-nanogel with LC₅₀ values of 27 and 32 μg/mL showed promising efficacies against Anopheles stephensi larvae. Besides, the efficacy of EGEO-nanogel (IC₅₀ 187 μg/mL) was significantly more potent than EGEO-nanoemulsion (IC₅₀ 3732 μg/mL) against Staphylococcus aureus. However, no significant difference was observed in the efficacy of EGEO-nanoemulsion and EGEO-nanogel against Pseudomonas aeruginosa. Natural components, straightforward preparation, and proper efficacy are some of the advantages of EGEO-nanogel; it could be considered for further consideration against other pathogens and mosquito larvae.

1. Background

Eucalyptus globulus (Family Myrtaceae) is a fast-growing evergreen and magnificent tree cultivated worldwide [1]. Its extracts have been traditionally used for tuberculosis, bacterial diarrhea, respiratory infections, joint pain, and similar cases [2, 3]. Moreover, the antioxidant activity of E. globulus and other Eucalyptus species, including E. citriodora, E. camaldulensis, E. microtheca, and E. sargentii, has been reported [4, 5]. Besides, the antibacterial effects of its essential oil (EGEO) on multidrug-resistant microorganisms have been confirmed [6–8]. For instance, EGEO affects Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus [6, 9]. Moreover, S. aureus and P. aeruginosa are opportunistic pathogens that cause acute and chronic hospital-acquired and respiratory tract infections [10, 11].

About 241 million malaria cases were identified worldwide in 2020, of which 627,000 people lost their lives [12]. Anopheles stephensi mosquitoes, as a domestic species, are one of the most important malaria vectors [13]. Insecticides have maintained a proven and effective tool for malaria control [13]. However, physiological and behavioral resistance in mosquito vectors such as A. stephensi is now a great threat to human health [14]. Therefore, using nonchemical or biopesticides (such as EO-based pesticides) to protect the environment and prevent mosquito resistance is a promising way to combat malaria vectors [15]. For example, the larvicidal activity of E. camaldulensis EO with an LC₅₀ value of 397.75 ppm against A. stephensi has been reported [16]. Furthermore, experimental laboratory data showed that E. tereticornis EO inhibited the pupal and adult activity of A. stephensi and showed nearly 100% mortality rate at the highest dose (160 ppm) [17].

Nanoemulsions with small droplets, optical transparency, and long-term physical stability (without coagulation, deposition, or biphasic) are easily absorbed by microorganisms. Therefore, their bioavailability is high and their efficiency increased compared to the nonnano state [18–20]. Despite the mentioned advantages, topical usage of nanoemulsions is challenging due to their low viscosity. In recent years, this challenge has been met by gelling the nanoemulsions, while the benefits of the nanoemulsion still exist [21, 22].

In this study, the chemical composition of EGEO was first investigated. After that, nanoemulsion and nanogel containing EGEO (EGEO-nanoemulsion and EGEO-nanogel) were prepared. Finally, their antibacterial and larvicidal activities against P. aeruginosa, S. aureus, and A. stephensi were investigated.

2. Methods

2.1. Materials

Standard species of P. aeruginosa and S. aureus (ATCC 27853 and 25923) were obtained from the Pasteur Institute of Iran. A. stephensi late 3^rd or early 4^th instar larvae were provided by Urmia University of Medical Sciences (Iran). Mosquitos were reared and kept at 29 ± 2°C with 70 ± 5% humidity. E. globulus EO bark’ EO was purchased from Tabib Daru pharmaceutical company (Iran) (TaDa 1729). Carboxymethylcellulose (CMC: C4888) was bought from Sigma–Aldrich (USA). Mueller Hinton agar (103872), Mueller Hinton broth (110293), and Tween 20 (817072) were bought from Merck Chemicals (Germany).

2.2. GC-MS Procedure

EO constituents were analyzed by a GC-MS system (Santa Clara, CA, USA) using a 6890 gas chromatography and a 5975 mass selective detector (Agilent Technologies, USA). The column was an HP-5MS silica-fused (length, 30 m; internal diameter, 0.25 mm; film thickness, 0.25 mm; stationary phase, 5% phenyl, and 95% methyl polysiloxane). The column temperature profile was as follows: 40°C (fixed for 1 min), then raised to 250°C at the rate of 3°C min⁻¹ and held for 60 minutes. The temperatures of the injection port and detector were set at 250 and 230°C, respectively. Helium (99.999%) was used as a carrier gas. The septum purge and column flow rate were fixed at 6 mL/min and 1 mL/min, respectively. Mass spectra were taken at full scan mode at 50–550 m/z with 70 eV ionization energy. Van den Dool and Kratz formula was applied to calculate the retention indices of n-alkanes [23]. The identification of the EO components was approached by combining the temperature programmed retention indices and mass spectra from ADAMS and the NIST 17 mass spectra library. Besides, relative abundances were obtained by peak area normalization [24, 25].

2.3. Preparation and Characterizations of EGEO-Nanoemulsion and EGEO-Nanogel

The nanoemulsions were prepared using the spontaneous method as reported in our previous study [26]. The EGEO (2% v/v) was first mixed (2000 rpm, 5 minutes) with different amounts of Tween 20 (2–6% v/v) at room temperature. Distilled water was then added drop wisely until reached 5000 μL (final volume) and stirred for 40 minutes (room temperature). Finally, prepared nanoemulsions were subjected to a DLS-type apparatus for size analysis. EGEO-nanoemulsion with optimal values (droplet size <200 nm and droplet size distribution (SPAN) <1) [27] was selected for further investigation, including preparation of EGEO-nanogel, antibacterial tests, and antilarval bioassays.

The optimum EGEO-nanoemulsion was gelified as follows. Then, CMC (3.5% w/v) was first added to the EGEO-nanoemulsion and the mixture was then stirred at 2000 rpm for 4 h at room temperature to complete the gelation process. The viscosity of EGEO-nanogel at shear rates of 0.1–10 1/s was analyzed by the rheometer apparatus (Anton Paar, model MCR-302, Austria) under atmospheric pressure at 25°C. Moreover, nanoemulsion (-oil) and nanogel (-oil) were also prepared using the same method, only without E. globulus EO.

ATR-FTIR analysis was used for the investigation of the loading of EGEO in EGEO-nanoemulsion and EGEO-nanogel. Spectra of EGEO, nanoemulsion (-oil), nanogel (-oil), EGEO-nanoemulsion, and EGEO-nanogel were recorded in the wavenumber range of 400–4000 cm⁻¹ [28]. The samples were subjected to the device (Tensor II model, Bruker Co, Germany) without any sample processing.

Furthermore, EGEO-nanoemulsion stability was checked using three tests. First, EGEO-nanoemulsion was 30 min centrifuged (14,000 g) at −4, +4, and +25°C, 30 min. Second, EGEO-nanoemulsion was subjected to freeze-thaw cycle tests; samples were placed for 48 h at −20°C (freezer) and room temperature for six successive periods. Third, EGEO-nanoemulsion was subjected to heating-cooling cycle tests; samples were placed for 48 h at +45°C (Bain–Marie) and room temperature for six successive periods. After each test, samples were visually checked for sedimentation, creaming, or biphasic. Moreover, EGEO-nanogel samples were stored at 4 and 26°C for six months and then were visually checked for sedimentation, creaming, or biphasic [29].

2.4. Larvicidal Bioassays

Larvicidal bioassays of EGEO-nanoemulsion and EGEO-nanogel were carried out according to the WHO recommended process [30]. Glass beakers containing 200 mL no-chlorine water and 25 larvae of A. stephensi were first prepared. The larvicidal effects of the EGEO-nanoemulsion and EGEO-nanogel were then investigated at 12.5, 25, 50, 100, and 150 μg/mL. After 24 h exposure, larval mortality rates were recorded. The control and blank groups were treated with ethanol and nanoemulsion (-oil) and nanogel (-oil).

2.5. Antibacterial Tests

The antibacterial properties of EGEO-nanoemulsion and EGEO-nanogel were investigated using the ATCC100 method [22]. First, aliquots of samples were sequentially diluted in 5 cm plate plates containing each bacterial suspension (2 × 10⁵ CFU/mL in the Mueller Hinton broth) to adjust final concentrations of 1250, 2500, and 5000 μg/mL. Then, treated plates were incubated at 37°C for 24 h and 10 μL-microbial suspensions were cultured on the Mueller Hinton agar plates and incubated for 24 h. The number of bacterial colonies forming units (CFU) was counted, and growth (%) was calculated by the following formula: (CFU sample/CFU control) × 100.

Furthermore, MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values are determined in semiquantitative assays such as disc or well diffusion methods. This study used ATTCC100 as a quantitative method; it investigated antibacterial effects, % bacterial growth, or % bacterial growth inhibitory, at different concentrations. The IC₅₀ value is measured this way, as calculated in this study.

2.6. Statistical Analyses

Three replicates were carried out for all tests and final values were reported as mean ± SD. The final values for all samples were compared with SPSS software using one-way ANOVA with a confidence interval of 95%. Turkey’s test evaluated statistical differences between groups. Besides, IC₅₀ (half-maximal inhibitory concentration) and LC₅₀ (Lethal concentration 50) values were calculated using CalcuSyn software (Free version, BIOSOFT, UK).

3. Results

3.1. Ingredients of EGEO

Eleven identified compounds in EGEO using GC-MS analysis are listed in Table 1. Five major compounds are 1,8-cineole (49.53%), α-pinene (15.33%), trans-pinocarveol (4.32%), aromadendrene (3.95%), and globulol (3.69%).

3.2. Prepared EGEO-Nanoemulsion and EGEO-Nanogel

The ingredients of five prepared EGEO-nanoemulsions and their size analysis are listed in Table 2. Sample No. 5 with droplet size (176 ± 8) and SPAN (0.97) showed the best size characteristics; its DLS profile is shown in Figure 1(a). Besides, the viscosity of the EGEO-nanogel at examined shear rates (0.01–100 1/s) is entirely consistent with the Carreau–Yasuda regression (Figure 1(b)). This well-known empirical equation has been used for nonNewtonian fluids such as biopolymer and polymeric solutions, emulsions, and protein solutions [31, 32].

(a)

(b)

Moreover, no creaming, sedimentation, and phase separation were observed in EGEO-nanoemulsions after all stability tests, including centrifugation (at −4, +4, +25°C), freeze-thaw cycles, and heating-cooling cycles. Besides, EGEO-nanogel did not show any biphasic or phase separation after six months of storage at 4 and 26°C. Therefore, the stability of EGEO-nanoemulsion and EGEO-nanogel was confirmed.

3.3. ATR-FTIR Spectra

The ATR-FTIR spectra of EGEO, nanoemulsion (-oil), EGEO-nanoemulsion, nanogel (-oil), and EGEO-nanogel are presented in Figure 2. In the ATR-FTIR spectrum of EGEO, broadband at about 3450 cm⁻¹ can be related to the OH group. The characteristic bands at 2880, 2921, and 2966 cm⁻¹ are attributed to CH stretching vibration, and the bands at about 1682 and 1644 cm⁻¹ can correspond to C=C stretching vibration in olefin. The strong peak at 1446 cm⁻¹ is allocated to symmetrical bending in the plane of C-H bonds and the strong band at 1375 cm⁻¹ can be related to CH₃ deformation. The bands at 1079 and 1214 cm⁻¹ corresponded to symmetric and asymmetric stretching of the C-O-C group. A sharp and strong band at 984 cm⁻¹ can be attributed to symmetrical bending out of the CH₂ plane.

In ATR-FTIR spectra of nanoemulsion (-oil), the broad peak at about 3200–3600 cm⁻¹ is attributed to OH stretching vibration due to hydrogen bonding between tween and water. The band at 2923 cm⁻¹ corresponds to the C-H stretching. The characteristic peak at 1732 cm⁻¹ is related to C=O stretching due to the carbonyl group in tween 20. Besides, the characteristic absorption at around 1457 cm⁻¹ exhibited CH₂ bending. Finally, the strong and characteristic band at 1087 cm⁻¹ is attributed to C-O stretching.

The ATR-FTIR spectrum of EGEO-nanoemulsion displayed the broad and characteristic band at around 3200–3700 cm⁻¹ can be related to OH groups in tween 20, H₂O, and EO that lead to hydrophilic interaction. The peak at about 2923 cm⁻¹ is related to C-H stretching due to EO and tween 20. The bands at 2341 and 2359 can be related to CO₂ and the band at 1733 cm⁻¹ is attributed to the carbonyl group in Tween 20. The band at 1457 cm⁻¹ can be related to CH₃ bending in EO and Tween 20. Totally between 400 and 4000 cm⁻¹, the intensities of the EGEO-nanoemulsion were higher than those in nanoemulsion (-oil), which is a sign of successful loading of EGEO in EGEO-nanoemulsion.

In the ATR-FTIR nanogel (-oil) spectrum, broadband in the region 3200–3600 cm⁻¹ can be related to OH due to hydrogen bonding. The peaks at about 2923 and 2855 cm⁻¹ are related to C-H stretching due to CMC. The characteristic band at 1734 cm⁻¹ related to C=O stretching is due to the carbonyl group in Tween 20 and CMC. The characteristic band at about 1577 cm⁻¹ is attributed to the carboxylate group’s asymmetric stretching. Besides, the peak at about 1417 cm⁻¹ corresponds to CMC’s symmetric stretching of the carboxyl group. Finally, the strong band at about 1081 cm⁻¹ can be attributed to C-O stretching vibration.

In the ATR-FTIR spectrum of the EGEO-nanogel, a broadband in the region 3200–3500 cm⁻¹ corresponded to OH due to hydrogen bonding in CMC, Tween 20, and the EO. The band at around 2923 cm⁻¹ is related to C-H stretching due to the EO, Tween 20, and CMC. The band at 1734 cm⁻¹ showed C=O stretching that exhibited the carbonyl group in the EO, Tween 20, and CMC. The sharp and strong band at 1080 cm⁻¹ corresponded to C-O stretching. In the presence of CMC, the carboxylate band at 1579 cm⁻¹ demonstrated intramolecular H-bonding between Tween 20 and CMC. The physical interaction between the surface –OH of the Tween 20 and the –OH groups of the CMC molecule led to the consumption of a small amount of –OH groups. The appearance of the other bands in EGEO and nanogel (-oil) confirmed the successful loading of EGEO in the prepared EGEO-nanogel

3.4. Antibacterial Effects

The antibacterial activities of EGEO-nanoemulsion and EGEO-nanogel against S. aureus are illustrated in Figure 3. As details show, the efficacy of EGEO-nanogel was more potent than EGEO-nanoemulsion at all examined concentrations, including 1250 μg/mL , 2500 μg/mL , and 5000 μg/mL . Interestingly, about 80% of bacterial growth was reduced after treatment with 5000 μg/mL EGEO-nanogel and EGEO-nanoemulsion. Besides, nanoemulsion (-oil) and nanogel (-oil) did not affect the growth of bacteria. Furthermore, EGEO-nanogel with IC₅₀ 187 μg/mL was significantly more potent than EGEO-nanoemulsion (Table 3).

The antibacterial activities of EGEO-nanoemulsion and EGEO-nanogel P. aeruginosa are depicted in Figure 4. There is no significant difference observed between the efficacy of EGEO-nanogel and EGEO-nanoemulsion. Besides, their efficacy on P. aeruginosa was less than S. aureus; only 20% of bacterial growth was reduced after treatment with 5000 μg/mL EGEO-nanogel and EGEO-nanoemulsion. Besides, the nanoemulsion (-oil) and nanogel (-oil) did not affect the growth of P. aeruginosa. As efficacies of EGEO-nanogel and EGEO-nanoemulsion at the highest concentration (5000 μg/mL) were less than 50%, their IC₅₀ values were not determined (Table 3).

3.5. Larvicidal Effects

The larvicidal activities of EGEO-nanoemulsion and EGEO-nanogel against A. stephensi are demonstrated in Figure 5. A dose-response effect was observed in the mortality rate of A. stephensi. The efficacy of EGEO-nanoemulsion was significantly more potent than EGEO-nanogel at concentrations of 12.5 and 50 μg/mL , however, the efficacy of EGEO-nanogel at a concentration of 100 μg/mL was significantly more potent than EGEO-nanoemulsion . Interestingly, perfect larval mortality was observed at two concentrations of EGEO-nanogel (100 and 150 μg/mL). However, as summarized in Table 3, the LC₅₀ values of EGEO-nanogel and EGEO-nanoemulsion were not significantly different (32 and 27 μg/mL). Moreover, nanoemulsion (-oil) did not affect larvae and nanogel (-oil) with 6% mortality had a negligible effect on larvae.

4. Discussion

Microbial infections and mosquito-borne diseases are still major public health challenges. S. aureus is one of the most important pathogens of food-borne diseases and community-associated infections worldwide. It is resistant to harsh environmental conditions and is highly stable in different temperatures (7 to 48.5°C), pH (4.2 to 9.3), and 15% NaCl concentrations [33, 34]. Besides, P. aeruginosa plays a major role in increased morbidity and mortality rates in respiratory diseases like cystic fibrosis. Moreover, antibiotic resistance in P. aeruginosa due to low permeability in the outer membrane has created many therapeutic challenges [35].

Furthermore, excessive use of insecticides has led to resistance in mosquito populations and environmental pollution [36, 37]. The development of natural medicine and insecticides is thus crucial. EOs with a wide range of biological activities such as antibacterial, antioxidant, anticancer, and antilarval effects are a great source for this purpose [38, 39]. However, their efficacy and stability should be improved for practical application. Nowadays, nanostructured-loaded EOs are considered a promising approach [40]. Nanostructures can also increase their solubility and improve stability and effectiveness [41, 42].

EGEO in the present study was used as a natural antibacterial and larvicide. Therefore, its ingredients were first identified using GC-MS analysis. 1,8-cineole was the most abundant component, followed by α-pinene (15.33%), trans-pinocarveol (4.32%), aromadendrene (3.95%), and globulol (3.69%). These findings were consistent with the literature that 1,8-cineole and α-pinene were introduced as two major components of EGEO or other Eucalyptus spp. EOs [43, 44]. For example, 1,8-cineole was the major ingredient in eight Eucalyptus species’ essential oils from Tunisia [44]. Differences in the levels of major compounds may be due to genetic effects or harvester place.

Furthermore, some studies have been published on the antibacterial effects of eucalyptus extract on E. coli, S. aureus, and P. fluorescens [45–47]. Besides, it has been confirmed that inhaling eucalyptus extracts benefit non/infectious respiratory disorders, such as bronchitis, asthma, and chronic obstructive pulmonary disease [48, 49]. Moreover, EGEO is an immune stimulant with antiinflammatory, antioxidant, and analgesic effects [48, 49]. Some reports on its nanoformulated states were also reported. For example, its nanoemulsion showed wound healing potential without skin irritation in Wistar rats [50]. Moreover, larvicidal effects of nanoemulsion of eucalyptus oil were reported against A. stephensi; a 98% mortality rate was obtained at 250 ppm [51]. However, we could not find any report on nanogel containing EGEO. The efficacy of EGEO-nanogel was more potent than the EGEO-nanoemulsion agent in the current study due to better stability.

5. Conclusions

1,8-cineole, α-pinene, trans-pinocarveol, aromadendrene, and globulol were identified as five major components of E. globulus EO (EGEO). The antibacterial and larvicidal effects of nanoemulsion and nanogel containing the EO (EGEO-nanoemulsion and EGEO-nanogel) were investigated. The efficacy of EGEO-nanogel against S. aureus was significantly more potent than EGEO-nanoemulsion; bacterial growth after treatment with 2500 and 5000 μg/mL of EGEO-nanogel was reduced by more than 80%. Besides, 100% larval mortality was observed after treatment with 100 and 150 μg/mL EGEO-nanogel. The EGEO-nanogel could thus be considered for further investigations against other pathogens and mosquito larvae.

Abbreviations

EO:	Essential oil
GC-MS:	Gas chromatography–mass spectrometry
ATR-FTIR:	Attenuated total reflection-Fourier transform infrared
EGEO:	Eucalyptus globulus essential oil
EGEO-nanoemulsion:	Nanoemulsion-containing EGEO
EGEO-nanogel:	Nanogel-containing EGEO.

Data Availability

All data are available from the corresponding author on reasonable request.

Ethical Approval

This study has been approved by the ethical committee of Fasa University of Medical Sciences, Fasa, Iran (IR.FUMS.REC.1400.164).

Conflicts of Interest

The authors declare that they have no conflicts of interest in this study.

Authors’ Contributions

HA drafted the MS. AA performed antibacterial tests. SF performed larvicidal bioassays. EZ interpreted ATR-FTIR spectra. MJ prepared the nanoemulsion and nanogel. MO designed the study, analysed data, and revised the MS. All authors contributed to the drafting of the manuscript. All authors read and approved the final manuscript.

Acknowledgments

The study was supported by Fasa University of Medical Sciences supported this study, grant no. 400175.

References

D. R. Batish, H. P. Singh, R. K. Kohli, and S. Kaur, “Eucalyptus essential oil as a natural pesticide,” Ecological Management, vol. 256, no. 12, pp. 2166–2174, 2008.
View at: Publisher Site | Google Scholar
A. J. Sales and A. Shariat, “Synergistic effects of silver nanoparticles with ethanolic extract of Eucalyptus globules on standard pathogenic bacteria in vitro,” Tabari Biomedical Student Research Journal, vol. 2, no. 3, pp. 13–21, 2020.
View at: Publisher Site | Google Scholar
C. Cermelli, A. Fabio, G. Fabio, and P. Quaglio, “Effect of eucalyptus essential oil on respiratory bacteria and viruses,” Current Microbiology, vol. 56, no. 1, pp. 89–92, 2008.
View at: Publisher Site | Google Scholar
A. Ghaffar, M. Yameen, S. Kiran et al., “Chemical composition and in-vitro evaluation of the antimicrobial and antioxidant activities of essential oils extracted from seven Eucalyptus species,” Molecules, vol. 20, no. 11, pp. 20487–20498, 2015.
View at: Publisher Site | Google Scholar
J. Safaei-Ghomi, M. Ghadamib, and H. Batooli, “Bioactivity of methanol extracts of Eucalyptus sargentii maiden cultivated in Iran,” Dig Nanomat Biostruct, vol. 5, pp. 859–863, 2010.
View at: Google Scholar
R. G. Bachir and M. Benali, “Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus,” Asian Pacific Journal of Tropical Biomedicine, vol. 2, no. 9, pp. 739–742, 2012.
View at: Publisher Site | Google Scholar
V. Aleksic Sabo and P. Knezevic, “Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: a review,” Industrial Crops and Products, vol. 132, pp. 413–429, 2019.
View at: Publisher Site | Google Scholar
D. Ben Hassine, M. Abderrabba, Y. Yvon et al., “Chemical composition and in vitro evaluation of the antioxidant and antimicrobial activities of Eucalyptus gillii essential oil and extracts,” Molecules, vol. 17, no. 8, pp. 9540–9558, 2012.
View at: Publisher Site | Google Scholar
B. R. Ghalem and B. Mohamed, “Antibacterial activity of essential oil of north west Algerian Eucalyptus camaldulensis against Escherichia coli and Staphylococcus aureus,” J Coast Life Med, vol. 2, no. 10, pp. 799–804, 2014.
View at: Publisher Site | Google Scholar
C. Cigana, I. Bianconi, R. Baldan et al., “Staphylococcus aureus impacts Pseudomonas aeruginosa chronic respiratory disease in murine models,” The Journal of Infectious Diseases, vol. 217, no. 6, pp. 933–942, 2018.
View at: Publisher Site | Google Scholar
A. Lupo, M. Haenni, and J. Y. Madec, “Antimicrobial resistance in acinetobacter spp. and Pseudomonas spp,” Microbiology Spectrum, vol. 6, no. 3, 2018.
View at: Publisher Site | Google Scholar
D. O. Oyewola, E. G. Dada, S. Misra, and R. Damaševičius, “A novel data augmentation convolutional neural network for detecting malaria parasite in blood smear images,” Applied Artificial Intelligence, vol. 36, pp. 1–22, 2022.
View at: Publisher Site | Google Scholar
H. Vatandoost, A. Raeisi, A. Saghafipour, F. Nikpour, and J. Nejati, “Malaria situation in Iran: 2002–2017,” Malaria Journal, vol. 18, no. 1, pp. 200–207, 2019.
View at: Publisher Site | Google Scholar
M. Weill, G. Lutfalla, K. Mogensen et al., “Insecticide resistance in mosquito vectors,” Nature, vol. 423, no. 6936, pp. 136-137, 2003.
View at: Publisher Site | Google Scholar
D. Kocher and A. Riat, “Larvicidal potential of Eucalyptus globulus oil against Anopheles stephensi,” Int J Mosq Res, vol. 6, pp. 24–26, 2019.
View at: Google Scholar
S. M. Medhi, S. Reza, K. Mahnaz et al., “Phytochemistry and larvicidal activity of Eucalyptus camaldulensis against malaria vector, Anopheles stephensi,” Asian Pacific Journal of Tropical Medicine, vol. 3, no. 11, pp. 841–845, 2010.
View at: Publisher Site | Google Scholar
S. Senthilnathan, “The use of Eucalyptus tereticornis Sm.(Myrtaceae) oil (leaf extract) as a natural larvicidal agent against the malaria vector Anopheles stephensi Liston (Diptera: Culicidae),” Bioresource Technology, vol. 98, no. 9, pp. 1856–1860, 2007.
View at: Publisher Site | Google Scholar
B. Sundararajan, A. K. Moola, K. Vivek, and B. R. Kumari, “Formulation of nanoemulsion from leaves essential oil of Ocimum basilicum L. and its antibacterial, antioxidant and larvicidal activities (Culex quinquefasciatus),” Microbial Pathogenesis, vol. 125, pp. 475–485, 2018.
View at: Publisher Site | Google Scholar
O. Koul, S. Walia, and G. Dhaliwal, “Essential oils as green pesticides: potential and constraints,” Biopestic Int, vol. 4, no. 1, pp. 63–84, 2008.
View at: Google Scholar
R. Badihi, A. Mahmoudi, M. R. Sazegar, and K. Nazari, “A study on co-modification of MSNs with some transition metals and polyethyleneimine (PEI) as a versatile strategy for efficient delivery of short oligonucleotides,” Chemical Papers, 2022.
View at: Publisher Site | Google Scholar
G. Roozitalab, Y. Yousefpoor, A. Abdollahi, M. Safari, F. Rasti, and M. Osanloo, “Antioxidative, anticancer, and antibacterial activities of a nanoemulsion-based gel containing Myrtus communis L. essential oil,” Chemical Papers, 2022.
View at: Publisher Site | Google Scholar
H. Qasemi, Z. Fereidouni, J. Karimi et al., “Promising antibacterial effect of impregnated nanofiber mats with a green nanogel against clinical and standard strains of Pseudomonas aeruginosa and Staphylococcus aureus,” Journal of Drug Delivery Science and Technology, vol. 66, Article ID 102844, 2021.
View at: Publisher Site | Google Scholar
H. Van Den Dool and P. D. Kratz, “A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography,” Journal of Chromatography A, vol. 11, 1963.
View at: Google Scholar
R. P. Adams, Identification of Essential Oil Components by Gas Chromatography/mass Spectrometry, Allured Publishing Corporation Carol Stream, 2007.
W. E. Wallace, W. Ji, D. V. Tchekhovskoi, K. W. Phinney, and S. E. Stein, “Mass spectral library quality assurance by inter-library comparison,” Journal of the American Society for Mass Spectrometry, vol. 28, no. 4, pp. 733–738, 2017.
View at: Publisher Site | Google Scholar
M. Osanloo, S. Firooziyan, A. Abdollahi et al., “Nanoemulsion and nanogel containing Artemisia dracunculus essential oil; larvicidal effect and antibacterial activity,” BMC Research Notes, vol. 15, no. 1, p. 276, 2022.
View at: Publisher Site | Google Scholar
A. Ghanbariasad, A. Valizadeh, S. N. Ghadimi, Z. Fereidouni, and M. Osanloo, “Nanoformulating Cinnamomum zeylanicum essential oil with an extreme effect on Leishmania tropica and Leishmania major,” Journal of Drug Delivery Science and Technology, vol. 63, Article ID 102436, 2021.
View at: Publisher Site | Google Scholar
D. L. Pavia, G. M. Lampman, G. S. Kriz, and J. A. Vyvyan, Introduction to Spectroscopy, Cengage Learning, Boston, MA, USA, 2014.
N. Abedinpour, A. Ghanbariasad, A. Taghinezhad, and M. Osanloo, “Preparation of nanoemulsions of mentha piperita essential oil and investigation of their cytotoxic effect on human breast cancer lines,” BioNanoScience, vol. 11, no. 2, pp. 428–436, 2021.
View at: Publisher Site | Google Scholar
W. H. Organization, Guidelines for Laboratory and Field Testing of Mosquito Larvicides, World Health Organization, Geneva, Switzerland, 2005.
R. B. Bird, R. C. Armstrong, and O. Hassager, Dynamics of Polymeric Liquids. Vol. 1: Fluid Mechanics, Wiley, Hoboken, NJ, USA, 1987.
R. Avazmohammadi and P. Ponte Castañeda, “The rheology of non-dilute dispersions of highly deformable viscoelastic particles in Newtonian fluids,” Journal of Fluid Mechanics, vol. 763, pp. 386–432, 2015.
View at: Publisher Site | Google Scholar
J. R. Fitzgerald, “Livestock-associated Staphylococcus aureus: origin, evolution and public health threat,” Trends in Microbiology, vol. 20, no. 4, pp. 192–198, 2012.
View at: Publisher Site | Google Scholar
J. Kadariya, T. C. Smith, and D. Thapaliya, “Staphylococcus aureus and staphylococcal food-borne disease: an ongoing challenge in public health,” BioMed Research International, vol. 2014, Article ID 827965, 9 pages, 2014.
View at: Publisher Site | Google Scholar
K. Streeter and M. Katouli, “Pseudomonas aeruginosa: a review of their pathogenesis and prevalence in clinical settings and the environment,” Infection, Epidemiology and Medicine, vol. 2, no. 1, pp. 25–32, 2016.
View at: Publisher Site | Google Scholar
A. Hanafi-Bojd, M. Abbasi, M. R. Yaghoobi-Ershadi et al., “Resistance status of main malaria vector, Anopheles stephensi Liston (Diptera: Culicidae) to insecticides in a malaria Endemic Area, Southern Iran,” Asian Pac J Trop Med, vol. 12, no. 1, pp. 43–48, 2019.
View at: Publisher Site | Google Scholar
S. Yared, A. Gebressielasie, L. Damodaran et al., “Insecticide resistance in Anopheles stephensi in Somali Region, eastern Ethiopia,” Malaria Journal, vol. 19, no. 1, pp. 180–187, 2020.
View at: Publisher Site | Google Scholar
F. Bakkali, S. Averbeck, D. Averbeck, and M. Idaomar, “Biological effects of essential oils–a review,” Food and Chemical Toxicology, vol. 46, no. 2, pp. 446–475, 2008.
View at: Publisher Site | Google Scholar
S. Noorpisheh Ghadimi, N. Sharifi, and M. Osanloo, “The leishmanicidal activity of essential oils: a systematic review,” J HerbMed Pharmacol, vol. 9, no. 4, pp. 300–308, 2020.
View at: Publisher Site | Google Scholar
F. Esmaili, A. Sanei-Dehkordi, F. Amoozegar, and M. Osanloo, “A review on the use of essential oil-based nanoformulations in control of mosquitoes,” Biointerface Res Appl Chem, vol. 11, no. 5, pp. 12516–12529, 2021.
View at: Publisher Site | Google Scholar
R. K. Thapa, G. M. Khan, K. Parajuli-Baral, and P. Thapa, “Herbal medicine incorporated nanoparticles: advancements in herbal treatment,” Asian j biomed pharm sci, vol. 3, no. 24, pp. 7–14, 2013.
View at: Google Scholar
A. Trifan, S. V. Luca, H. Greige-Gerges, A. Miron, E. Gille, and A. C. Aprotosoaie, “Recent advances in tackling microbial multidrug resistance with essential oils: combinatorial and nano-based strategies,” Critical Reviews in Microbiology, vol. 46, no. 3, pp. 338–357, 2020.
View at: Publisher Site | Google Scholar
K. Sebei, F. Sakouhi, W. Herchi, M. L. Khouja, and S. Boukhchina, “Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves,” Biological Research, vol. 48, no. 1, pp. 7–5, 2015.
View at: Publisher Site | Google Scholar
A. Elaissi, Z. Rouis, N. A. B. Salem et al., “Chemical composition of 8 eucalyptus species’ essential oils and the evaluation of their antibacterial, antifungal and antiviral activities,” BMC Complementary and Alternative Medicine, vol. 12, no. 1, pp. 81–15, 2012.
View at: Publisher Site | Google Scholar
M. Salari, G. Amine, M. Shirazi, R. Hafezi, and M. Mohammadypour, “Antibacterial effects of Eucalyptus globulus leaf extract on pathogenic bacteria isolated from specimens of patients with respiratory tract disorders,” Clinical Microbiology and Infections, vol. 12, no. 2, pp. 194–196, 2006.
View at: Publisher Site | Google Scholar
Cock, “Antimicrobial activity of Eucalyptus major and Eucalyptus baileyana methanolic extracts,” The Internet Journal of Microbiology, vol. 6, no. 1, p. 31, 2009.
View at: Google Scholar
M. R. Ammer, S. Zaman, M. Khalid et al., “Optimization of antibacterial activity of Eucalyptus tereticornis leaf extracts against Escherichia coli through response surface methodology,” Journal of Radiation Research and Applied Sciences, vol. 9, no. 4, pp. 376–385, 2016.
View at: Publisher Site | Google Scholar
A. E. Sadlon and D. W. Lamson, “Immune-modifying and antimicrobial effects of Eucalyptus oil and simple inhalation devices,” Alternative Medicine Review, vol. 15, no. 1, pp. 33–47, 2010.
View at: Google Scholar
S. Xiao, P. Cui, W. Shi, and Y. Zhang, “Identification of essential oils with activity against stationary phase Staphylococcus aureus,” BMC complement med ther, vol. 20, no. 1, pp. 99–10, 2020.
View at: Publisher Site | Google Scholar
S. Sugumar, V. Ghosh, M. J. Nirmala, A. Mukherjee, and N. Chandrasekaran, “Ultrasonic emulsification of eucalyptus oil nanoemulsion: antibacterial activity against Staphylococcus aureus and wound healing activity in Wistar rats,” Ultrasonics Sonochemistry, vol. 21, no. 3, pp. 1044–1049, 2014.
View at: Publisher Site | Google Scholar
S. Sugumar, S. Clarke, M. Nirmala, B. Tyagi, A. Mukherjee, and N. Chandrasekaran, “Nanoemulsion of eucalyptus oil and its larvicidal activity against Culex quinquefasciatus,” Bulletin of Entomological Research, vol. 104, no. 3, pp. 393–402, 2014.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2022 Hiva Alipanah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

617

Downloads

571

Citations