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Influenza Research and Treatment
Volume 2010 (2010), Article ID 976084, 3 pages
http://dx.doi.org/10.1155/2010/976084
Case Report

Shedding of the Pandemic Swine-Origin Influenza A Virus (H1N1) after Oseltamivir Administration

1Unit of Viral Genome Regulation, Institut Pasteur of Shanghai, The Key Laboratory of Virology and Immunology, Chinese Academy of Sciences, 411 Hefei Road, Shanghai 200025, China
2Emerging Infectious Diseases, Institut Pasteur of Shanghai, The Key Laboratory of Virology and Immunology, Chinese Academy of Sciences, 411 Hefei Road, Shanghai 200025, China
3Institut Pasteur in Cambodia, 5 Monivong Boulevard, P.O. Box 983, Phnom Penh, Cambodia
4Choju Medical Institute, Fukushimura Hospital, 19-4 Azanakayama, Noyori-cho, Toyohashi, Aichi 441-8124, Japan
5Infectious Disease Regulation Project, Tokyo Metropolitan Institute of Medical Sciences, 1-6, Kamikitazawa 2-chome, Setagaya-ku, Tokyo156-8506, Japan

Received 1 October 2010; Revised 5 November 2010; Accepted 15 November 2010

Academic Editor: Daniel Roberto Perez

Copyright © 2010 Leiyun Weng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We analyzed the virus shedding of an oseltamivir-treated patient who had been infected with the pandemic swine-origin influenza A (H1N1) virus which had an oseltamivir-sensitive neuraminidase. The virus was isolated from the pharyngeal swabs of the patient using MDCK cells, and the virus genome RNA was detected in the same samples both by real-time RT-PCR and RT-PCR. The virus was isolated until 44 h after oseltamivir administration although the virus genome was detected until one day after oseltamivir treatment was stopped. Due to their high sensitivity, RT-PCR and real-time RT-PCR may cause misdiagnosis by detection of viral genome which does not infect, and classical virus isolation and clinical symptoms are recommended for the evaluation of oseltamivir treatment.