Research Article
A Novel Vaccine Using Nanoparticle Platform to Present Immunogenic M2e against Avian Influenza Infection
Figure 6
SDS-PAGE of SAPN Constructs. (a) Purification of Mono-M2e. (1) Molecular weight marker protein ladder (New England Biolabs, Ipswich, MA). (2) Flow through. (3) wash with lysis buffer. (4) Wash with high-phosphate buffer. (5) pH 6.3 wash. (6) pH 5.9 wash. (7) pH 4.3 wash. (8) Wash with 250 mM imidazole buffer. (9) Wash with 500 mM imidazole buffer. (10) Wash with 1000 mM imidazole buffer. (b) Purification of Tetra-M2e. (1) Molecular weight marker protein ladder. (2) Flow through. (3) pH 6.3 wash. (4) pH 5.9 wash. (5) pH 4.3 wash. (6) Wash with 100 mM imidazole buffer. (7) Wash with 250 mM imidazole buffer. (8) Wash with 500 mM imidazole buffer. (9) Wash with 1000 mM imidazole buffer fraction # 1. (10) Wash with 1000 mM imidazole buffer fraction no. 15.
