Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler

Table 2

Detection and isolation of influenza H3N2 virus in concentrated virus collection media.

		RT-PCR detection and virus isolation
		Site 1	Site 2	Site 3	Site 4	Site 5	Site 6	Site 7
SKC BioSampler		D^a, VI^b	D, VI	D, VI	—, —	—, —	—, —	—, —
PCIS
Filter position	Cutoff size
Stage A	>2.5 𝜇m	D, VI	—, —	—, —	—, —	—, —	—, —	—, —
Stage B	1.0–2.5 𝜇m	D, —	—, —	—, —	—, —	—, —	—, —	—, —
Stage C	0.5–1.0 𝜇m	D, VI	D, VI	D, VI	—, —	—, —	—, —	—, —
Stage D	0.25–0.5 𝜇m	D, VI	D, VI	D, VI	—, —	—, —	—, —	—, —
After-filter	<0.25 𝜇m	D, VI	D, —	D, VI	—, —	—, —	—, —	—, —

aD: detection of viral RNA by RT-PCR.
^bVI: virus isolation.