Effect of the PB2 and M Genes on the Replication of H6 Influenza Virus in Chickens

Figure 1

Representative characteristics of plaque formed on MDCK cell cultures. Typical large plaques were induced by the parental W312 strain (a). The plaque size of each reassortant virus with ST-PB1 (d), ST-PA (e), ST-NP (g), ST-NA (h), and ST-NS (j) was similar to that of the W312 strain. The reassortant virus with ST-PB2 (c) and ST-M (i) formed obviously smaller plaque compared with the other reassortants. Countable clear plaques were not detected when parental ST strain (b) and the virus harbored the ST-HA gene (f) resulting in forming the same characteristics in the cells as that of ST strain.

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