Table 3: Summary of validation parameters.

Sr. no.ParameterLAFDOP

(1)Absorption maxima (nm)279284
(2)Linearity range (μg/mL)10–1005–40
(3)Standard regression equationy = 0.818x + 0.208y = 1.552x − 0.036
(4)Correlation coefficient ( 𝑟 2 ) 0. 9990.997
(5)Molar absorptivity260811480
(6)A (1%, 1 cm) 𝜆 1 = 6 0 . 5 2 𝜆 1 = 2 7 9
𝜆 𝟐 = 5 6 . 0 8 𝜆 2 = 3 0 4
(7)Accuracy (% recovery ± SD)99.18
(8)Precision (% CV)0.5684
(9)Limit of quantitation (μg/mL)2.080.479
(10)Limit of detection (μg/mL)6.111.45