Table 1: System suitability results (precision, intermediate precision, and robustness) for MP, KT, and MF.

ParametersMPKTMF
>3000≤2.0≤2.0>3000≤2.0≤2.0>3000≤2.0≤2.0
% *RT % *RT % *RT

Precision41901.10.272.30142701.00.173.09554721.00.444.665
Interprecision44521.10.222.30642681.10.273.10154761.00.334.674
1.7 mL/min flow rate38811.10.292.04037811.10.142.73350071.00.834.137
1.3 mL/min flow rate49231.10.222.63748121.10.193.54161491.00.325.322
55°C column temp.44551.10.242.27843651.10.313.05856071.00.254.486
45°C column temp.45141.10.392.34141991.10.253.13555801.00.614.844
Mobile phase pH 6.742891.10.292.30242711.10.073.09555481.01.014.659
Mobile phase pH 6.343161.10.172.30342611.10.163.08655651.00.564.644
+3.5% Acetonitrile44221.10.352.27141701.10.332.94054801.01.024.282
−3.5% Acetonitrile43231.10.592.36844971.00.173.34556411.00.695.242

: plate count, : tailing factor, : relative standard deviation, RT: retention time, *determined on five values.