Table 3: Method precision, intermediate precision result, LOD, LOQ evaluation, and linearity data for MP, KT, and MF.

ParameterMPKTMF

Precision day-1 ( )
(% Assay ± SD; % RSD)
100.5 ± 0.48; 0.4899.7 ± 0.43; 0.43100.2 ± 0.76; 0.75
Intermediate precision day-2 ( )
(% Assay ± SD; % RSD)
100.0 ± 0.32; 0.32100.4 ± 0.17; 0.17101.3 ± 0.59; 0.58
LOD ( g/mL)0.0330.2040.123
LOQ ( g/mL)0.120.670.42
Linearity range ( g/mL)0.12–15.20.67–149.40.42–7.6
Correlation coefficient0.99990.99990.9999
Intercept ( )1485.841415.816−1614.022
Slope ( )72985.06617217.39635584.579
Bias at 100% response−0.2020.024−0.914