Table 3: ANOVA results for experimental responses in the OA16 (45) matrix.

FactorDOFaSum of squaresVariance -ratiobPure sum of squares % PCc

pH (A)3 0.21 5.99
% surfactant (B)3 0.514 16.3
Ionic strength (C) 3 0.343 10.49
Temperature (D) 3 0.353 10.85
Organic solvent volume (µL)3 1.333 44.21
Error16 12.16

Total31 100

Degrees of freedom. b , critical value is 3.24 ( ). cPercent of contribution.