Table 5: Robustness study of developed HPTLC method.

ParametersSDa(%) RSD

Mobile phase composition (4.9 : 3.9 : 1 : 1, 5.1 : 4.1 : 0.9 : 0.9)1.311.36
Saturation time (  min)0.500.50
Development distance (  cm)1.181.16
Scanning time interval (1 h, 2 h, 3 h, 4 h, 5 h, and 6 h)0.150.15
Wavelength change (  nm)0.720.73
Time from spotting to chromatography (0, 20, 40, and 60 min)0.520.52
Mobile phase volume (  mL)1.581.58

replicates for concentration of 800 ng/band.