- About this Journal
- Abstracting and Indexing
- Aims and Scope
- Article Processing Charges
- Articles in Press
- Author Guidelines
- Bibliographic Information
- Citations to this Journal
- Contact Information
- Editorial Board
- Editorial Workflow
- Free eTOC Alerts
- Publication Ethics
- Submit a Manuscript
- Subscription Information
- Table of Contents
ISRN Analytical Chemistry
Volume 2013 (2013), Article ID 734027, 11 pages
Use of Two Sulfonphthalein Dyes for the Sensitive and Selective Extraction-Free Spectrophotometric Assay of Albendazole in Bulk Drug and in Tablets
Department of Chemistry, University of Mysore, Mysore, Karnataka 570 006, India
Received 20 April 2013; Accepted 14 May 2013
Academic Editors: A. Bouklouze and A. Przyjazny
Copyright © 2013 Nagaraju Swamy and Kanakapura Basavaiah. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Albendazole (ALB) is a potent benzimidazole anthelmintic used in the treatment of human intestinal helmintiasis as well as of hytatid cysts and neurocysticercosis. Two rapid, simple, sensitive, and selective spectrophotometric methods are presented for the determination of ALB in pharmaceuticals. The methods are based on the formation of dichloromethane soluble 1 : 1 ion-pair complexes (ALB : dye) formed between ALB and sulfonphthalein dyes, bromophenol blue BPB, (method A) and bromothymol blue BTB, (method B). The complexes formed were measured directly (without extraction) at 445 nm (method A) and 460 nm (method B). The experimental conditions were optimized and the systems obey Beer’s law for 1.5–21.0 and 2.0–32.0 μg mL−1 ALB for method A and method B respectively. The molar absorptivity and Sandell's sensitivity were calculated to be L mol−1 cm−1 and 0.0209 ng cm−2, and L mol−1 cm−1 and 0.0350 ng cm−2 using BPB and BTB, respectively. The limits of detection and quantification were calculated to be 0.01 and 0.03, and 0.16, and 0.49 μg mL−1 using BPB and BTB, respectively. The relative standard values for intra-day and inter-day precision were less than 3%, and the accuracy was better than 3% for both methods.
Albendazole (ALB), chemically known as methyl-5-(propyl thio)-2-yl benzimidazole methyl carbamate , is widely used as an anthelmintic having a wide spectrum of activity . The drug is official in British Pharmacopoeia , which describes a potentiometric titration with perchloric acid in formic acid-acetic acid medium. Quantitative determination of ALB in dosage forms has received wide attention and several methods have been reported and include titrimetry in nonaqueous medium [4, 5], redox titrimetry [6–12], UV spectrophotometry [13–17], spectrofluorimetry [18, 19], voltammetry [20–22], high performance liquid chromatography [23–27], and high performance thin layer chromatography [28, 29].
Several visible spectrophotometric methods, both direct and indirect, are found in the literature for the determination of ALB in pharmaceuticals. Basavaiah et al.  in their methods treated ALB with a known excess of NBS in HCl medium, and the unreacted oxidant was reduced by iron(II) and the resulting iron(III) was complexed with thiocyanate or tiron offering two sensitive methods. Two more indirect methods  involving NBS-metol-sulfanilamide and NBS-celesteine blue as chromogenic systems have been proposed by Sastry et al. Several other reaction schemes involving chloramine-T-methyl orange or indigocarmine , chloramine-T-metol-sulfanilic acid , NBS-methyl orange or indigocarmine , N-chlorosuccinimide-iron(II)-thiocyanate , NaIO4-KBr-methyl orange or indigocarmine , and perchloric acid-crystal violet  are also found in the literature for the indirect spectrophotometric assay of ALB in its dosage forms.
Only a few direct spectrophotometric methods are reported for ALB. Zarapkar and Deshpande  have determined the drug in tablets and syrups based on the reduction of Folin-Ciocalteu reagent to a blue coloured chromogen which was measured at 700 nm. The charge-transfer reaction of ALB with π-acceptor chloranilic acid in alcoholic acetone medium was used by Zhao et al.  for the determination of the drug in tablets. Based on similar reaction and employing chloranilic acid and 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) as π-acceptors, determination of ALB in tablets has been reported by Refat et al. . Ion-pair reaction involving ALB and acidic dyes has been the basis of extractive spectrophotometric assay of ALB [35–37]. Sane et al.  have described four procedures based on this reaction using bromocresol green, bromophenol blue, bromothymol blue, and bromophenol red as ion-pair reagents in acidic buffer medium. The ion-pair complexes formed were extracted into chloroform and measured at 420 nm. The methods were applied to tablets and syrup. Thymol blue and six alizarin derivatives  are the other ion-pair reagents used for the assay of ALB. Sastry et al.  have devised extractive spectrophotometric methods for the estimation of some benzimidazole anthelmintics including ALB in pharmaceuticals based on ion-pair reaction and employing some acidic dyes.
All the indirect spectrophotometric methods cited above [4, 6, 7, 10–12, 30, 31] are cumbersome involving multireagents and multistep reactions and, hence prone to inaccuracy and imprecision. On the other hand, the reported direct spectrophotometric methods [33, 34] are less sensitive with the molar absorptivity value being <1 × 104 L mol−1 cm−1. The methods based on ion-pair reaction [35–37], though sensitive, involve tedious and time consuming extraction step besides being critically dependent on pH of the aqueous phase and the aqueous-organic phases ratio. Additionally, incomplete extraction of the analyte may lead to erratic results. In an attempt to overcome these shortcomings associated with extractive spectrophotometric methods, several workers in recent years have devised extraction-free spectrophotometric methods for several pharmaceutical substances [38–45] based on ion-pair reaction and employing some sulfonphthalein dyes. These new procedures are free from critical pH adjustment and laborious liquid-liquid extraction step and involve simple mixing the drug and dye solutions in organic solvent and measuring the absorbance of the resulting ion-pair complex at wavelength of maximum absorption.
The present work presents two simple, rapid, sensitive, and selective spectrophotometric methods for ALB based on ion-pair reaction and using bromophenol blue (BPB) and bromothymol blue (BTB) as ion pair reagents. The striking feature of these methods is the absence of the extraction step, and the methods are absolutely free from critical experimental variables, unlike the direct and indirect spectrophotometric methods cited earlier. They were demonstrated to be highly precise and accurate besides being robust and rugged.
A Systronics model 166 digital spectrophotometer (Systronics, Ahmedabad, Gujarat, India) equipped with 1 cm matched quartz cells was used for all absorbance measurements.
Pharmaceutical grade ALB (99.7 percent pure) was received as a gift from Cipla India, Ltd., Mumbai, India and used as received. Alworm-400 (Medopharm Ltd., Malur, India), ABD-400 (Intas Pharmaceuticals, Ltd., Ahmedabad, India,) and Bandy-400 (Mankind Pharmaceuticals, Ltd., New Delhi, India) tablets were purchased from local commercial sources.
2.3. Reagents and Chemicals
All reagents used were of analytical reagent grade, and HPLC grade organic solvents were used throughout the investigation.
A 0.025% bromophenol blue (Loba Chemie Pvt. Ltd., Mumbai, India) and 0.1% bromothymol blue (Qualigens Fine Chemicals, Mumbai, India) solutions were prepared separately in dichloromethane (DCM, Merck, Mumbai, India; specific gravity 1.32) and used in method A and method B, respectively.
2.4. Standard ALB Solution
A stock standard solution containing 100 μg mL−1 ALB was prepared by dissolving 10 mg of pure drug in dichloromethane and diluting to mark with the same solvent in a 100 mL calibrated flask. This was diluted appropriately with the same solvent to obtain working concentration of 30 μg mL−1 for method A and 40 μg mL−1 ALB for method B.
2.5. Assay Procedures
2.5.1. Method A (Using Bromophenol Blue, BPB)
Different aliquots of 0.25, 0.5, 1.0, 2.0, 3.0, and 3.5 mL of standard ALB solution (30 μg mL−1) were accurately transferred to 5 mL standard flasks using a microburette, and the total volume was adjusted to 3.5 mL by adding adequate quantity of DCM. One mL of 0.025% BPB was added to each flask and the mixture was diluted to the volume with DCM and mixed well. The absorbance of each solution was measured at 445 nm against a reagent blank after 5 min.
2.5.2. Method B (Using Bromothymol Blue, BTB)
Aliquots of 0.25, 0.5, 1.0, 2.0, 3.0, 3.5, and 4.0 mL of standard ALB solution (40 μg mL−1) were measured accurately and added to 5 mL calibrated flasks using a microburette, and the total volume was adjusted to 4.0 mL with DCM. One mL of 0.1% BTB was added to each flask and the mixture was diluted to the volume with DCM and mixed well. After 5 min, the absorbance of each solution was measured at 460 nm against a reagent blank prepared simultaneously.
A standard graph was prepared by plotting absorbance against concentration, and the unknown concentration was read from the graph or computed from the regression equation derived using Beer’s law data.
2.6. Procedure for Tablets
The content of ten tablets each containing 400 mg ALB was weighed accurately and grounded into a fine powder. An accurately weighed quantity equivalent to 10 mg of ALB was transferred into a 100 mL calibrated flask and dissolved in 60 mL of DCM. The content was shaken thoroughly for 15–20 min, diluted to the mark with DCM, mixed well, and filtered using a Whatman no. 42 filter paper. The first 10 mL portion of the filtrate was discarded, and a suitable aliquot of the filtrate (100 μg mL−1) was diluted to obtain working concentration of 30 μg mL−1 in method A and 40 μg mL−1 in method B with DCM and was analysed by taking an appropriate volume in both methods.
2.7. Procedure for the Selectivity Study
Selectivity was evaluated by using placebo blank and synthetic mixture analysis. A placebo blank, consisting of starch (20 mg), acacia (25 mg), hydroxyl cellulose (20 mg), sodium citrate (30 mg), talc (20 mg), magnesium stearate (25 mg), and sodium alginate (20 mg), was prepared by thorough mixing and its solutions described under procedure for tablets and then were subjected to analysis.
A synthetic mixture was prepared by adding 10 mg of pure ALB to ~10 mg of the above mentioned placebo blank, and the mixture was homogenized. Following the procedure used for tablets, the synthetic mixture solution was prepared, and a suitable quantity was subjected to analysis by both methods () after appropriate dilution.
3. Results and Discussion
3.1. Absorption Spectra
The reaction of BPB or BTB with ALB results in the formation of intense yellow coloured product. The absorption of coloured products was recorded at 360–520 nm against the corresponding reagent blank solutions. The resulting coloured ion pair complexes showed maximum absorbance at 445 nm and 460 nm for ALB-BPB (a) and ALB-BTB (b), respectively. Thus, all absorbance measurements were thus made at these wavelengths (Figure 1).
3.2. Reaction Pathway
ALB forms ion-pair complexes with dyes such as BPB and BTB, since it contains a tertiary amino group which is protonated in the solvent medium used. Chemically, the structure of ALB features its basic nature. This structure suggests the possibility of utilizing an anionic dye as chromogenic reagent. In dichloromethane, ALB does not absorb in the visible region. In contrast, when a solution of BPB/BTB in dichloromethane is added to the drug solution, an intense yellow coloured product is produced immediately. This is due to an opening of lactoid ring of the dye and subsequent formation of quinoid group . It is supposed that the two tautomers are present in equilibrium but due to strong acidic nature of the sulfonic acid group, the quinoid body must predominate. Finally, protonated ALB forms ion-pair with the anionic dye. The possible reaction pathway is shown in Schemes 1 and 2. Anionic dye as BPB or BTB forms ion-pair complex with positively charged drug. Each drug-dye ion-pair complex molecule, with two oppositely charged ions, behaves as a single unit held together by an electrostatic force of attraction (Schemes 1 and 2).
3.3. Optimisation of Reaction Conditions
3.3.1. Effect of Reagent Concentration
The effect of the dye concentration on the intensity of the colour developed at selected wavelengths was studied by measuring the absorbances of solutions containing a fixed concentration of ALB and different amounts (0.5–2.5 mL) of the respective dye of 0.025% BPB and 0.1% BTB solution. Maximum colour intensity was achieved with 1 mL of dye in each case and excess dye did not affect the absorbance of the complex (Figure 2).
3.3.2. Effect of Solvent
In order to select the suitable solvent for ion-pair complex formation, the reaction of ALB with BPB and BTB was carried out in different solvents such as DCM, chloroform, acetonitrile, methanol, 1,2-dichloroethane, 1,4-dioxane, and benzene. The DCM was found to be ideal solvent for both methods A and B (Figure 3). Similarly, the effect of the diluting solvent was studied for both methods and the results showed that DCM was best suited for both methods with low blank absorbance and high complex absorbance.
3.3.3. Effect of Reaction Time and Stability of Colour
The addition of the dye solution resulted in an immediate full colour development at ambient temperature and the formed ion-pair complexes were stable for at least 1.5 h in method A and half an hour in method B. The reaction was found to be complete quantitative when the reaction mixture was allowed to stand for 5 min.
3.3.4. Stoichiometric Relationship
Job’s method of continuous variations of equimolar solutions was employed to establish the stoichiometry of formed ion-pair complexes. Solutions equivalent to 1.13 × 10−4 M ALB and BPB were prepared in DCM in method A, and 3.02 × 10−4 M ALB and BTB were prepared in method B by dissolving the calculated quantities in DCM. A series of solutions was prepared in which the total volume of ALB and reagent was kept at 3.0 mL in 5 mL calibrated flask. The solutions were well mixed; the volume was completed to the mark with DCM. The absorbance of the resulting solution was measured after 10 min at the respective wavelengths of maximum absorbance against the organic solvent as blank in the respective methods. The resulting graph (Figure 4) shows that the interaction occurs on an equimolar basis (1 : 1 reaction stoichiometry), owing to the presence of one basic nitrogen containing group in the ALB molecule.
The conditional stability constant () of the ion-association complex was calculated from the continuous variations data using the following equation : where and are the observed maximum absorbance and the absorbance value when all the drug present is associated, respectively. is the molar concentration of drug at the maximum absorbance and is the stoichiometry with which BPB/BTB ion associates with ALB ion. The values were found to be 7.58 and 6.62 for method A and method B, respectively.
3.4. Method Validation
3.4.1. Linearity, Sensitivity, Limits of Detection, and Quantification
A linear correlation was found between absorbance at and concentration of ALB in the ranges given in Table 1. The graphs are described by the regression equation: where = absorbance of 1 cm layer of solution, = intercept, = slope, and = concentration in μg mL−1. Regression analysis of the Beer’s law data using the method of least squares was made to evaluate the slope (), intercept (), and correlation coefficient () for each system and the values are presented in Table 1. The optical characteristics such as Beer’s law limits, molar absorptivity, and Sandell’s sensitivity values  of all the three methods are also given in Table 1.
The limits of detection (LOD) and quantitation (LOQ) calculated according to ICH guidelines  using the following formulae: where is the standard deviation of blank absorbance values, and is the slope of the calibration plot are also presented in Table 1. The values of and Sandell’s sensitivity and LOD indicate the sensitivity of the proposed methods.
3.5. Accuracy and Precision
To evaluate the accuracy and precision of the proposed methods, pure ALB solution at three different concentration levels (within working range) was prepared and analyzed in seven replicates during the same day (intraday precision) and on five consecutive days (interday precision) and the results are presented in Table 2. The percentage relative error (RE %) was ≤ 2.18 which indicate that the accuracy of the methods is satisfactory. Percentage relative standard deviation (RSD %) for intraday was ≤2.25 and for interday was ≤2.35 indicating repeatability and usefulness of the proposed methods in the analysis.
In order to evaluate the selectivity of the proposed methods for the analysis of pharmaceutical formulations, the effect of the presence of common excipients was tested for possible interference in the assay by placebo blank and synthetic mixture analyses. The absorbance of the reaction product with the placebo blank was almost equal to the absorbance of the reagent blank suggesting no interference from the tablet excipients. The percent recoveries of ALB were and for methods A and B, respectively. This confirms the selectivity of the methods in the presence of the common tablet excipients.
3.7. Robustness and Ruggedness
To determine the robustness of the proposed methods, an important experimental variable, volume of reagent was slightly varied and the effect of this change on the absorbance of the ion-pair complex was studied. The results of this effect are presented in Table 3 and indicated that the proposed methods are robust. Method ruggedness was evaluated by performing the analysis following the general procedures by three different analysts and on three different spectrophotometers by the same analyst. From the % RSD values presented in Table 3, it is concluded that the proposed methods are rugged.
3.8. Application for Tablets
The proposed methods were applied to the determination of ALB in tablets. The results obtained were statistically compared with those of the official method  by applying Student’s -test for accuracy and -test for precision. The official method describes a potentiometric titration with perchloric acid in formic acid-acetic acid medium. As can be seen from Table 4, the calculated and values at 95% confidence level did not exceed the tabulated values of 2.78 and 6.39, respectively, for four degrees of freedom. The results showed that there is no difference between the proposed methods and the official method with respect to accuracy and precision.
3.9. Recovery Study
To further assess the accuracy of the methods, recovery experiments were performed by applying the standard-addition technique. The recovery was assessed by determining the agreement between the measured standard concentration and added known concentration to the sample. The test was done by spiking the preanalysed tablet powder with pure ALB at three different levels 50, 100, and 150% of the content present in the tablet powder (taken) and the total was found by the proposed methods. Each test was repeated three times. In all the cases, the recovery percentage values ranged between 99.34% and 101.7% with standard deviation in the range 1.31%–2.81%. Closeness of the results to 100% showed the fairly good accuracy of the methods. The results are shown in Table 5.
Two spectrophotometric methods for the determination of albendazole in bulk drug and in tablets were developed and validated for accuracy, precision, linearity, robustness, and ruggedness. The methods employ more normal conditions than those previously reported and rely on well-characterised ion-pair formation reactions. Besides, the methods have the advantages of simplicity without involving heating or extraction step and high sensitivity. The proposed methods are rapid, simple and in addition offer advantages in determining ALB (in tablets), when extraction difficulties arise with other spectrophotometric methods based on ion-pair extraction (Table 6). Hence, the proposed methods could be adopted for quality control in pharmaceutical industries.
The authors express their gratitude to the quality control manager, Cipla India, Ltd., Mumbai, India, for gift sample of pure albendazole. The first author (Nagaraju Swamy) is grateful to the authorities of the University of Mysore, Mysore, India, for permission and facilities. The authors do not have any conflict of interests with the company name used in the paper.
- The Extra Pharmacopoeia, Royal Pharmaceutical Society of Great Britan, London, UK, 24th edition, 1989.
- L. Goodman and A. Gilman, The Pharmaceutical Basis of Therapeutics, Chapter 44, McMilan Publishing Company, New York, NY, USA, 7th edition, 1985.
- British Pharmacopoeia, The Stationary Office, London, UK, 2009.
- K. Basavaiah, P. Nagegowda, and V. Ramakrishna, “Titrimetric and spectrophotometric methods for the assay of albendazole in non-aqueous medium,” Proceedings of the National Academy of Sciences, India, vol. 74, no. 3, pp. 271–276, 2004.
- N. De Laurentis, M. A. Milillo, and S. Bruno, “Determination of albendazole as raw material and in tablets by nonaqueous titration with sodium methylate solution,” Revista de Farmacia e Bioquimica da Universidade de Sao Paulo, vol. 33, no. 1, pp. 23–27, 1997.
- K. Basavaiah and H. C. Prameela, “Use of an oxidation reaction for the quantitative determination of albendazole with chloramine-T and acid dyes,” Analytical Sciences, vol. 19, no. 5, pp. 779–784, 2003.
- K. Basavaiah and H. C. Prameela, “Two simple methods for the estimation of albendazole and its dosage forms using chloramine-T,” Farmaco, vol. 58, no. 7, pp. 527–534, 2003.
- K. Basavaiah and H. C. Prameela, “Kinetic and titrimetric determination of albendazole using bromate and methyl orange,” Indian Journal of Pharmaceutical Sciences, vol. 67, no. 1, pp. 57–60, 2005.
- K. Basavaiah and H. C. Prameela, “Titrimetric and spectrophotometric determination of albendazole with bromate and methyl orange,” Oxidation Communications, vol. 27, no. 1, pp. 177–185, 2004.
- K. Basavaiah, V. Ramakrishna, and B. C. Somashekar, “Assay of albendazole using N-bromosuccinimide,” The Indian Pharmacist, vol. 5, no. 53, pp. 129–136, 2006.
- K. Basavaiah and P. Nagegowda, “Three new methods for the assay of albendazole using N-chlorosuccinimide,” Journal of Scientific and Industrial Research, vol. 63, no. 10, pp. 835–841, 2004.
- K. Basavaiah, V. Ramakrishna, B. C. Somashekar, and U. R. Anilkumar, “Sensitive titrimetric and spectrophotometric methods for the assay of albendazole in pharmaceuticals using sodium periodate,” Analytical Chemistry Indian Journal (TSI), vol. 2, no. 5, pp. 159–166, 2006.
- A. C. Tella, O. M. Olabemiwo, M. O. Salawu, and G. K. Obiyenwa, “Developing a spectrophotometric method for the estimation of Albendazole in solid and suspension forms,” International Journal of Physical Sciences, vol. 5, no. 4, pp. 379–382, 2010.
- S. C. Mandal, M. Bhattacharyya, A. K. Maity, B. K. Gupta, and S. K. Ghosal, “Determination of albendazole in tablet formulations by U.V. spectrophotometric method,” Indian Drugs, vol. 29, no. 7, pp. 323–324, 1992.
- M. M. Fregonezi-Nery, M. M. Baracat, É. R. M. Kedor-Hackmann, and R. M. Pinheiro, “Determination of albendazole in oral suspension,” Analytical Letters, vol. 34, no. 8, pp. 1255–1263, 2001.
- O. Amit, M. Kondawar, P. Shital, S. Nazarkar, N. Manohar, and H. Narkhede, “Validated UV-spectrophotometric method for the estimation of albendazole in tablet dosage form,” Journal of Pharmacy Research, vol. 3, no. 6, pp. 1355–1357, 2010.
- C. Soto, D. Contreras, S. Orellana, J. Yañez, and M. I. Toral, “Simultaneous determination of albendazole and praziquantel by second derivative spectrophotometry and multivariated calibration methods in veterinary pharmaceutical formulation,” Analytical Sciences, vol. 26, no. 8, pp. 891–896, 2010.
- S. Küçükkolbaşi, B. Gündüz, and E. Kiliç, “Development of a spectrofluorimetric method for determination of albendazole in tablets,” Analytical Letters, vol. 41, no. 1, pp. 104–118, 2008.
- G. Y. Zhao, H. Wu, S. L. Dong, and L. M. Du, “Study on the inclusion interaction of methylated-β-cyclodextrins with albendazole by spectrofluorimetry and its application,” Chinese Chemical Letters, vol. 19, no. 8, pp. 951–954, 2008.
- A. A. Zuhri, A. I. Hussein, M. J. Musmar, and S. Yaish, “Adsorptive stripping voltammetric determination of albendazole at a hanging mercury drop electrode,” Analytical Letters, vol. 32, no. 15, pp. 2965–2975, 1999.
- A. L. Santos, R. M. Takeuchi, M. P. Mariotti, M. F. De Oliveira, M. V. B. Zanoni, and N. R. Stradiotto, “Study of electrochemical oxidation and determination of albendazole using a glassy carbon-rotating disk electrode,” Farmaco, vol. 60, no. 8, pp. 671–674, 2005.
- M. F. De Oliveira and N. R. Stradiotto, “Voltammetric assay of albendazole in pharmaceutical dosage forms,” Analytical Letters, vol. 34, no. 3, pp. 377–387, 2001.
- R. T. Sane, R. S. Samant, M. D. Joshi et al., “HPLC determination of albendazole in pharmaceuticals,” Indian Drugs, vol. 26, no. 9, pp. 494–496, 1989.
- B. Liawruangrath and S. Liawrungrath, “HPLC method for the determination of albendazole,” ACGC Chemical Research Communications, vol. 8, pp. 45–50, 1998.
- Y. S. R. Krishnaiah, K. Latha, R. S. Karthikeyan, and V. Satyanarayana, “HPLC method for the estimation of albendazole in pharmaceutical dosage forms,” Acta Ciencia Indica, vol. 27, no. 4, p. 161, 2002.
- Z. Atkoşar and G. Altiokka, “The determination of albendazole by flow injection analysis method using UV-detection and HPLC method in suspensions,” Journal of Liquid Chromatography and Related Technologies, vol. 29, no. 6, pp. 849–856, 2006.
- A. Waldia, S. Gupta, R. Issarani, and B. P. Nagori, “Validated liquid chromatographic method for simultaneous estimation of albendazole and ivermectin in tablet dosage form,” Indian Journal of Chemical Technology, vol. 15, no. 6, pp. 617–620, 2008.
- S. J. Varghese, P. Vasanthi, and T. K. Ravi, “Simultaneous densitometric determination of ivermectin and albendazole by high-performance thin-layer chromatography,” Journal of Planar Chromatography, vol. 24, no. 4, pp. 344–347, 2011.
- O. I. Abd El-Sattar, N. M. El-Abasawy, S. A. Abdel-Razeq, M. M. Ismail, K. A. Attia, and N. S. Rashed, “Stability indicating method for determination of albendazole and fenbendazole in the presence of their degradates,” Egyptian Journal of Biomedical Sciences, vol. 18, pp. 105–109, 2005.
- K. Basavaiah, K. Tharpa, R. Prasad, S. G. Hiriyanna, and K. B. Vinay, “Simple, sensitive and rapid spectrophotometric determination of albendazole based on redox and complex formation using n-bromosuccinimide,” Proceedings of the Indian National Science Academy, vol. 75, no. 1, pp. 1–6, 2009.
- C. S. P. Sastry, V. A. N. Sarma, U. V. Prasad, and C. S. R. Lakshmi, “N-bromosuccinimide as an analytical reagent for the spectrophotometric determination of benzimidazole anthelmintics,” Indian Journal of Pharmaceutical Sciences, vol. 59, no. 4, pp. 161–164, 1997.
- S. S. Zarapkar and P. M. Deshpande, “Colorimetric determination of albendazole and its dosage forms,” Indian Journal of Pharmaceutical Sciences, vol. 50, no. 5, pp. 296–297, 1988.
- G. Zhao, H. Li, Y. Liu, and Y. Wang, “Application of charge-transfer reaction between albendazole and chloranilic acid,” Fenxi Huaxue, vol. 29, no. 4, pp. 389–400, 2001.
- M. S. Refat, G. G. Mohamed, and A. Fathi, “Spectrophotometric determination of albendazole drug in tablets: spectroscopic characterization of the charge-transfer solid complexes,” Chinese Journal of Chemistry, vol. 29, no. 2, pp. 324–332, 2011.
- R. T. Sane, D. P. Gangal, R. V. Tendolkar, K. D. Ladage, and R. M. Kothurkar, “An extractive colorimetric method for the determination of albendazole from pharmaceutical preparations,” Indian drugs, vol. 26, no. 11, pp. 632–635, 1989.
- M. S. Kamel, B. N. Barsoum, and R. Sayed, “Spectrophotometric microdetermination of anthelmintic drug in pure form and pharmaceutical formulation by ion-pair complexation,” Journal of Applied Sciences Research, vol. 4, no. 10, pp. 1242–1248, 2008.
- C. S. P. Sastry, A. N. Sarma Varahabhatla, U. V. Prasad, and C. S. R. Lakshmi, “A note on the estimation of some benzimidazole anthelmintics in pharmaceutical preparations by ion-pair extraction method,” Indian Drugs, vol. 34, no. 2, pp. 102–104, 1997.
- H. Abdine, F. Belal, and N. Zoman, “Simple spectrophotometric determination of cinnarizine in its dosage forms,” Farmaco, vol. 57, no. 4, pp. 267–271, 2002.
- S. M. Al-Ghannam, “A simple spectrophotometric method for the determination of β-blockers in dosage forms,” Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 1, pp. 151–156, 2006.
- D. H. Manjunatha, S. M. T. Shaikh, K. Harikrishna, R. Sudhirkumar, P. B. Kandagal, and J. Seetharamappa, “Simple and sensitive spectrophotometric methods for the determination of acebutolol hydrochloride in bulk sample and pharmaceutical preparations,” Ecletica Quimica, vol. 33, no. 2, pp. 37–40, 2008.
- K. Kovács-Hadady and I. Fábián, “The determination of benzalkonium chloride in eye-drops by difference spectrophotometry,” Journal of Pharmaceutical and Biomedical Analysis, vol. 16, no. 5, pp. 733–740, 1998.
- N. Rajendraprasad, K. Basavaiah, and K. B. Vinay, “Sensitive and selective extraction-free spectrophotometric determination of Quetiapine fumarate in pharmaceuticals using the two sulphonphthalein dyes,” Journal of Preclinical and Clinical Research, vol. 4, no. 1, pp. 24–31, 2010.
- P. J. Ramesh, K. Basavaiah, O. Zenita Devi, and K. B. Vinay, “Simple and sensitive spectrophotometric assay of ofloxacin in pharmaceuticals based on ion-pair reaction,” Chemical Industry and Chemical Engineering Quarterly, vol. 16, no. 4, pp. 353–362, 2010.
- M. M. El-Kerdawy, M. A. Moustafa, S. M. El-Ashry, and D. R. El-Waseef, “Spectrophotometric determination of certain phenothiazines and their dosage forms using bromophenol blue,” Analytical Letters, vol. 26, no. 8, pp. 1669–1680, 1993.
- H. H. Abdine, “Spectrophotometric determination of cisapride using some sulfonphthalein dyes,” Alex Journal of Pharmacy Science, vol. 14, pp. 75–78, 2000.
- A. S. Douglas and M. W. Donald, Principels of Instrumental Analysis Holt, Rinhart and Winston, New York, NY, USA, 1971.
- N. Erk, “Extractive spectrophotometric methods for the novel antidepressant drug in bulk and pharmaceutical dosage forms by using bromthymol blue and bromcresol green,” Analytical Letters, vol. 36, no. 6, pp. 1183–1196, 2003.
- H. Zavis, D. Ludvik, K. Milan, S. Ladislaw, and V. Frantisck, Handbook of Organic Reagents in Inorganic Analysis, University of Aberdem, Ellis Horwood Limited, Chichester, UK, John Wiley & Sons, New York, NY, USA, 1976, Translated by Stanislav, K., Dr. Chalmers The Series and Translation Editor.
- International Conference on Hormonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R1), Complementary Guideline on Methodology dated 06 November 1996, incorporated in November 2005, London, UK.