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ISRN Analytical Chemistry
Volume 2013 (2013), Article ID 734027, 11 pages
http://dx.doi.org/10.1155/2013/734027
Research Article

Use of Two Sulfonphthalein Dyes for the Sensitive and Selective Extraction-Free Spectrophotometric Assay of Albendazole in Bulk Drug and in Tablets

Department of Chemistry, University of Mysore, Mysore, Karnataka 570 006, India

Received 20 April 2013; Accepted 14 May 2013

Academic Editors: A. Bouklouze and A. Przyjazny

Copyright © 2013 Nagaraju Swamy and Kanakapura Basavaiah. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Albendazole (ALB) is a potent benzimidazole anthelmintic used in the treatment of human intestinal helmintiasis as well as of hytatid cysts and neurocysticercosis. Two rapid, simple, sensitive, and selective spectrophotometric methods are presented for the determination of ALB in pharmaceuticals. The methods are based on the formation of dichloromethane soluble 1 : 1 ion-pair complexes (ALB : dye) formed between ALB and sulfonphthalein dyes, bromophenol blue BPB, (method A) and bromothymol blue BTB, (method B). The complexes formed were measured directly (without extraction) at 445 nm (method A) and 460 nm (method B). The experimental conditions were optimized and the systems obey Beer’s law for 1.5–21.0 and 2.0–32.0 μg mL−1 ALB for method A and method B respectively. The molar absorptivity and Sandell's sensitivity were calculated to be  L mol−1 cm−1 and 0.0209 ng cm−2, and  L mol−1 cm−1 and 0.0350 ng cm−2 using BPB and BTB, respectively. The limits of detection and quantification were calculated to be 0.01 and 0.03, and 0.16, and 0.49 μg mL−1 using BPB and BTB, respectively. The relative standard values for intra-day and inter-day precision were less than 3%, and the accuracy was better than 3% for both methods.