Table 3: Method robustness and ruggedness expressed as intermediate precision.

Method robustnessMethod ruggedness
Method ALB taken
(µg mL−1)
Parameters altered,
volume of BPB/BTB
mLa % RSD
( )
Interanalysts
(% RSD),
( )
Interinstruments
(% RSD),
( )

A6.0
12.0
18.0
2.16
1.80
1.94
2.49
1.84
2.02
2.52
1.92
2.14

B8.0
16.0
24.0
2.11
1.65
1.74
2.46
1.94
2.06
2.75
2.07
2.35

In both methods, the volumes of reagent were 1 ± 0.1 mL.