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ISRN Analytical Chemistry
Volume 2013 (2013), Article ID 861851, 7 pages
http://dx.doi.org/10.1155/2013/861851
Research Article

Spectrophotometric Determination of Bromhexine HCl in Pure and Pharmaceutical Forms

1Department of Chemistry, Jawaharlal Nehru Technology University, Hyderabad 500085, India
2Department of Chemistry, Nizam College, Hyderabad 500001, India

Received 8 June 2013; Accepted 3 July 2013

Academic Editors: E. Billiot, S. Göktürk, and D. Patra

Copyright © 2013 K. Susmitha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Five spectrophotometric methods for determination of bromhexine HCl have been developed, validated, and applied for the assay of the drug in pharmaceuticals. Methods A, B, and C are based on ion-pair complexation of drug, in acidic buffers, with triphenylmethane dyes, namely, bromothymol blue (BTB), bromophenol blue (BPB), and bromocresol green (BCG). The complexes are extracted into chloroform, and absorbance is measured at around 415 nm as function of concentration of the drug. The stoichiometry of the complex is found to be 1 : 1 in each case. Method D depends upon charge-transfer complexation of neutralized drug with iodine which produces iodide ion whose absorbance at 366 nm is measured as function of concentration of the drug. This complex too has 1 : 1 composition as determined by Job’s method. Method E is developed on the basis of oxidation of the drug with alkaline KMnO4 which generates green-colored manganate ion with 610 nm. As the intensity of green color increased with increasing time kinetics of the reaction is followed, and calibration curves are constructed by using initial rate and fixed time methods. Excellent recovery studies with high accuracy and precision indicate that the methods can be successfully used in industries for the assay of drug in pure form and pharmaceuticals.