Table 6: Method robustness.

ConditionModificationMean peak area ± SD*% RSDMean Rt ± SD% RSDMean theoretical plates ± SD*% RSDMean tailing factor ± SD% RSD

Temperature (°C)20147204 ± 105.00.882.01 ± 0.010.552976 ± 431.431.101 ± 0.0040.35
25147271 ± 157.21.092.12 ± 0.020.723067 ± 591.941.117 ± 0.0030.23
30147144 ± 156.31.521.99 ± 0.010.333119 ± 642.071.108 ± 0.0030.24

Mobile phase composition70 : 30147371 ± 147.30.842.01 ± 0.010.572979 ± 280.961.020 ± 0.0030.29
75 : 25147304 ± 260.81.322.12 ± 0.010.473040 ± 521.681.110 ± 0.0010.32
85 : 15147511 ± 295.11.682.00 ± 0.010.363119 ± 652.071.050 ± 0.0070.67

Flow rate0.25147307 ± 110.150.631.99 ± 0.0040.233079 ± 120.391.110 ± 0.0040.36
0.30147171 ± 163.70.422.11 ± 0.0050.243104 ± 80.161.110 ± 0.0040.34
0.35147211 ± 110.000.762.11 ± 0.06060.263129 ± 120.411.108 ± 0.0030.28

Wavelength218 nm147341 ± 163.710.342.12 ± 0.0030.143019 ± 240.811.124 ± 0.0050.45
220 nm147218 ± 215.480.382.11 ± 0.0030.123169 ± 260.841.117 ± 0.0030.23
222 nm147254 ± 58.590.482.12 ± 0.0040.173153 ± 300.951.106 ± 0.0050.46

  *Mean value of three determinations.