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ISRN Analytical Chemistry
Volume 2013 (2013), Article ID 964601, 7 pages
http://dx.doi.org/10.1155/2013/964601
Research Article

Effects of Enoant and Ischemia and Reperfusion on Lens Metabolites of Rats

1Istanbul Training and Research Hospital, Clinic of Ophthalmology, Fatih, 34098 Istanbul, Turkey
2Department of Physiology, Faculty of Medicine, Yeni Yüzyıl University, Topkapi, Istanbul, Turkey
3Istanbul University, Department of Neuroscience, Institute for Experimental Medicine, Istanbul, Turkey
4Department of Analytical Chemistry, Faculty of Pharmacy, Bezmialem Vakif University, Fatih, 34093 Istanbul, Turkey
5Faculty of Engineering and Natural Sciences, Sabancı University, Tuzla, Istanbul, Turkey

Received 3 July 2013; Accepted 29 July 2013

Academic Editors: A. Garcia Asuero and C. Y. Panicker

Copyright © 2013 Hülya Güngel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of the ischemia and reperfusion on the lens metabolites and the effect of a phytotherapeutic commercial product called “Enoant” (mixed polyphenol content) on the selected lens metabolites were investigated. For this aim, 30 Wistar rats were divided into three groups according to their diet and being subjected to ischemia. 10 of the rats as Group I were fed on dry diet; the other 10 (Group II) were fed on dry diet and drinking water with Enoant. At the end of 15 days period, both groups of rats were subjected to ischemia for 2 hours and reperfused. After another 15 days with their same diet, the rats were decapitated. The remaining 10 rats, which were not subjected to ischemia (Group III), were fed on dry diet only. 1HNMR spectroscopy was used for the determination of lens metabolites of each group of rats. The results obtained from the three groups have been compared statistically. The differences of metabolites were significant except pyruvate and succinate. Oral administration of Enoant revealed effects on increasing membrane stabilization, the antioxidant capacity, osmotic regulator molecule capacity, and sorbitol content of lens disturbed by ischemia. Enoant can be used where oxidative or osmotic stress is formed.