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ISRN Analytical Chemistry
Volume 2014 (2014), Article ID 351547, 6 pages
Research Article

Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Flavonoid Glycosides in Withania somnifera Aerial Parts

1R&D Centre, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Industrial Area, 19th K.M. Stone, Hosur Road, Bangalore 560100, India
2American Herbal Products Association, 8630 Fenton Street, Suite 918, Silver Spring, MD 20910, USA

Received 28 January 2014; Accepted 18 February 2014; Published 10 March 2014

Academic Editors: E. Gumienna-Kontecka and W. Lee

Copyright © 2014 Deepak Mundkinajeddu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Withania somnifera (L.) Dunal (Solanaceae) commonly known as ashwagandha, is an important plant in Ayurveda and is believed to increase longevity and vitality. The root is considered to be the medicinally important part of the plant as per classical texts and accordingly is the subject of most Pharmacopeial monographs. The aerial parts, being less expensive, are sometimes mixed with roots to prepare “standardized” extracts of W. somnifera, and in cases with false declaration of plant part used as roots on the certificate of analysis. The present study described a new, simple, accurate, and precise HPLC method for the simultaneous determination of flavonoid glycosides as unique constituents of the aerial parts, being absent in roots of the plant. The RSD for intra- and interday analyses was less than 2.5% and the recovery was 90–108%. The method was used to analyze samples of roots and aerial parts of the plant collected from India and Egypt. The samples of commercially available extracts of W. somnifera were also analyzed and many samples were found to contain flavonoid glycosides indicating a possible undeclared use of aerial parts in the extracts derived from roots in commercial practice.