Table 2: Results of validation of HPLC method.

Validation parameters123

Specificity
 Peak purity (%)>98>98>98
Linearity
 Conc. range, µg/mL8.82 to 564.308.55 to 547.209.11 to 583.30
0.99960.99960.9995
LOD
 µg/mL0.50.50.5
LOQ
 µg/mL1.51.51.5
Precision
 Interday precision
  (RSD), %1.451.581.22
 Intraday precision
  (RSD), %1.381.552.10
Accuracy: at three different concentrations in triplicate ( )
 Recovery (%)93–10792–10791–107