Table 2: Results of robustness study (OLP concentration, 10 μg mL−1).

Chromatographic conditionAlterationPeak area precisionRetention time precision
Mean area* ± SDSERSD, %Mean ± SDSERSD, %

Mobile phase flow rate (mL min−1)0.9606358 ± 0.00580.0030.0524.819 ± 0.0040.0230.073
1.0547577 ± 0.0000.0000.0004.364 ± 0.0030.0020.070
1.1498323 ± 0.0100.0060.1083.972 ± 0.0020.0020.038

Column temperature (°C)38546292 ± 0.0250.0140.2494.399 ± 0.0200.0110.464
40548214 ± 0.0060.0030.0574.364 ± 0.0010.0060.023
42548214 ± 0.0200.0120.1983.972 ± 0.0020.0060.023

Mean value of three injections.