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ISRN Analytical Chemistry
Volume 2014 (2014), Article ID 968314, 6 pages
http://dx.doi.org/10.1155/2014/968314
Research Article

The Liquid Chromatographic Determination of Chlorogenic and Caffeic Acids in Xu Duan (Dipsacus asperoides) Raw Herb

Herbal Analysis and Pharmacological Laboratories (HAPL), Centre for Complementary Medicine Research, University of Western Sydney, P.O. Box 1797, Penrith South DC, NSW 1797, Australia

Received 23 January 2014; Accepted 24 February 2014; Published 4 May 2014

Academic Editors: A. M. Haji Shabani and V. A. Lemos

Copyright © 2014 Jarryd L. Pearson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A validated analytical method is reported for the analysis of chlorogenic and caffeic acids in Xu Duan (Dipsacus asperoides) in the dried raw herb. The ground samples were extracted by ultrasonication in water and the extract was analysed by LC-PDA with identity confirmation by (+)ESI-MS/MS. A C18 column was used with a 0.1% aqueous formic acid : methanol gradient mobile phase. The analytes were quantified 325 nm. With the MS detector, using the chlorogenic acid precursor ion with m/z 354, ions with m/z 191, and 85 were produced. For caffeic acid the precursor ion with m/z 181, ions with m/z 163, 135, and 89 were produced. The amount of chlorogenic and caffeic acids in the raw herb was found to be 4.46 and 0.63 mg/g, respectively, and the method LOD was 0.13 and 0.02 mg/g, respectively.