Table 1: Effect of sublethal exposure (20% and 60% of 24 h and 96 h LC50) of bait formulations containing eugenol, ferulic acid, umbelliferone, and limonene on the activity of alkaline phosphatase (ALP) in the nervous tissue of the snail L. acuminata.

Treatment24 h LC5096 h LC50Withdrawal
20%60%20%60%60% (96 h LC50)

Control (Agar)2.75 ± 0.13 (100)2.25 ± 0.61 (100)
Control (a) Glu. + Meth.2.82 ± 0.51 (100)2.75 ± 0.32 (100)
Control (b) Star. + Meth.2.65 ± 0.32 (100)2.61 ± 0.16 (100)
Control (c) Glu. + Hist.2.75 ± 0.92 (100)2.64 ± 0.15 (100)
Control (d) Star. + Hist.2.65 ± 0.82 (100)2.31 ± 0.25 (100)
Glu. + Meth. + Eug.1.95 ± 0.06* (70.90)1.32 ± 0.12* (48.00)1.75 ± 0.23* (63.63)1.28 ± 0.72* (46.54)1.99 ± 0.72+ (88.44)
Glu. + Meth. + Fer.1.66 ± 0.25* (60.36)1.25 ± 0.07* (45.45)1.85 ± 0.36* (67.27)1.33 ± 0.20* (48.36)1.75 ± 0.26+ (77.77)
Glu. + Meth. + Umb.1.75 ± 0.11* (63.63)1.55 ± 0.20* (56.36)1.75 ± 0.13* (63.63)1.55 ± 0.09* (56.36)1.86 ± 0.23+ (82.66)
Glu. + Meth. + lim.1.25 ± 0.03* (45.45)1.03 ± 0.26* (37.45) 1.85 ± 0.77* (67.27)1.66 ± 0.21* (60.36)1.89 ± 0.73+ (84.00)
Star. + Meth. + Eug.1.98 ± 0.72* (72.00)1.70 ± 0.08* (61.81)1.65 ± 0.26* (60.00)1.60 ± 0.13* (58.18)1.82 ± 0.23+ (80.88)
Star. + Meth. + Fer.1.34 ± 0.06* (48.72)1.22 ± 0.38* (44.36)1.55 ± 0.26* (56.36)1.13 ± 0.07* (41.09)1.38 ± 0.07+ (61.33)
Star. + Meth. + Umb.1.68 ± 0.23* (61.09)1.38 ± 0.16* (50.18)1.40 ± 0.39* (50.90)1.28 ± 0.36* (46.54)1.55 ± 0.38+ (68.88)
Star. + Meth. + Lim.1.35 ± 0.72* (49.09)1.25 ± 0.07* (45.45)1.48 ± 0.21* (53.81)1.28 ± 0.77* (46.54)1.49 ± 0.24+ (66.22)
Glu. + hist. + Eug.1.85 ± 0.11* (67.27)1.63 ± 0.07* (59.27)1.53 ± 0.23* (55.63)1.48 ± 0.20* (53.81)1.65 ± 0.23+ (73.33)
Glu. + Hist. + Fer.1.79 ± 0.38* (65.09)1.58 ± 0.28* (57.45)1.51 ± 0.01* (54.90)1.31 ± 0.19* (47.63)1.52 ± 0.23+ (67.55)
Glu. + Hist. + Umb.1.62 ± 0.25* (58.90)1.35 ± 0.09* (49.09)1.68 ± 0.98* (61.09)1.36 ± 0.22* (49.45)1.49 ± 0.26+ (66.22)
Glu. + Hist. + Lim.1.48 ± 0.63* (53.81)1.25 ± 0.71* (45.45)1.60 ± 0.23* (58.18)1.25 ± 0.85* (45.81)1.51 ± 0.73+ (67.11)
Star. + Hist. + Eug.0.98 ± 0.23* (35.63)0.75 ± 0.03* (27.27)0.65 ± 0.75* (23.63)0.55 ± 0.32* (20.00)0.78 ± 0.23+ (34.66)
Star. + Hist. + Fer.1.23 ± 0.07* (44.72)1.11 ± 0.98* (40.36)1.05 ± 0.60* (38.18)0.99 ± 0.23* (36.00)1.20 ± 0.03+ (53.33)
Star. + Hist. + Umb.1.69 ± 0.08* (61.45)1.68 ± 0.23* (61.09)1.51 ± 0.23* (54.90)1.38 ± 0.75* (50.18)1.59 ± 0.23+ (70.66)
Star. + Hist. + Lim.1.42 ± 0.72* (51.63)1.24 ± 0.29* (45.09)1.48 ± 0.05* (53.81)1.28 ± 0.65* (46.54)1.55 ± 0.26+ (69.33)

Each value is mean ± SE of six replicates. Values in parentheses are percent change with control taken as 100%.
Concentration (w/v) has been expressed as final concentration in aquarium water. *Significant (P < 0.05) when t-test was applied in between treated and control groups and +in between 60% of 96 h LC50 and withdrawal group. Glu.: glucose; Star.: starch; Meth.: methionine; Hist.: histidine; Eug.: eugenol; Feb.: ferulic acid; Umb.: umbelliferone; Lim.: limonene.