Table 2: Effect of sublethal exposure (20% and 60% of 24 h and 96 h LC50) of bait formulations containing eugenol, ferulic acid, umbelliferone, and limonene on the activity of acetylcholinesterase (AchE) in the nervous tissue of the snail L. acuminata.

Treatment24 h LC 5096 h LC50Withdrawal
20%60%20%60%60% (96 h LC50)

Control (Agar)0.099 ± 0.002 (100)0.097 ± 0.007 (100)
Control (a) Glu. + Meth.0.098 ± 0.003 (100)0.097 ± 0.005 (100)
Control (b) Star. + Meth.0.097 ± 0.002 (100)0.098 ± 0.006 (100)
Control (c) Glu. + Hist.0.096 ± 0.005 (100)0.095 ± 0.001 (100)
Control (d) Star. + Hist.0.098 ± 0.004 (100)0.097 ± 0.006 (100)
Glu. + Meth. + Eug.0.075 ± 0.001* (75.75)0.063 ± 0.002* (63.63)0.05 ± 0.001* (58.58)0.051 ± 0.003* (51.51)0.067 ± 0.006+ (69.07)
Glu. + Meth. + Fer.0.065 ± 0.003* (65.65)0.051 ± 0.002* (51.51)0.086 ± 0.003* (86.86)0.065 ± 0.004* (65.65)0.078 ± 0.003+ (98.73)
Glu. + Meth. + Umb.0.073 ± 0.001* (73.73)0.068 ± 0.008* (68.68)0.070 ± 0.003* (70.70)0.055 ± 0.001* (55.55)0.063 ± 0.001+ (64.94)
Glu. + Meth. + lim.0.078 ± 0.002* (78.78)0.069 ± 0.001* (69.69)0.067 ± 0.002* (67.67)0.060 ± 0.003* (60.60)0.075 ± 0.002+ (77.31)
Star. + Meth. + Eug.0.055 ± 0.006* (55.55)0.050 ± 0.003* (50.50)0.058 ± 0.005* (58.58)0.049 ± 0.006* (49.49)0.055 ± 0.004+ (56.70)
Star. + Meth. + Fer.0.062 ± 0.004* (62.62)0.060 ± 0.008* (60.60)0.058 ± 0.001* (58.58)0.054 ± 0.003* (54.54)0.059 ± 0.003+ (60.82)
Star. + Meth. + Umb.0.072 ± 0.005* (71.71)0.065 ± 0.001* (65.65)0.062 ± 0.002* (62.62)0.057 ± 0.005* (57.57)0.062 ± 0.003+ (63.91)
Star. + Meth. + Lim.0.072 ± 0.008* (72.72)0.063 ± 0.004* (63.63)0.068 ± 0.003* (68.68)0.058 ± 0.002* (58.58)0.062 ± 0.001+ (63.91)
Glu. + hist. + Eug.0.057 ± 0.001* (57.57)0.054 ± 0.002* (54.54)0.055 ± 0.007* (55.55)0.050 ± 0.007* (50.50)0.058 ± 0.006+ (59.79)
Glu. + Hist. + Fer.0.082 ± 0.003* (82.82)0.075 ± 0.006* (75.75)0.069 ± 0.003* (69.69)0.061 ± 0.003* (61.61)0.069 ± 0.003+ (71.13)
Glu. + Hist. + Umb.0.070 ± 0.008* (70.70)0.068 ± 0.001* (68.68)0.069 ± 0.004* (69.69)0.066 ± 0.002* (66.66)0.070 ± 0.006+ (72.16)
Glu. + Hist. + Lim.0.069 ± 0.003* (69.69)0.066 ± 0.003* (66.66)0.065 ± 0.007* (65.65)0.060 ± 0.003* (60.60)0.006 ± 0.003+ (68.04)
Star. + Hist. + Eug.0.057 ± 0.006* (57.57)0.055 ± 0.004* (55.55)0.055 ± 0.003* (55.55)0.051 ± 0.005* (51.51)0.057 ± 0.008+ (58.76)
Star. + Hist. + Fer.0.060 ± 0.003* (60.60)0.059 ± 0.003* (59.59)0.058 ± 0.002* (58.58)0.055 ± 0.001* (55.55)0.058 ± 0.002+ (59.79)
Star. + Hist. + Umb.0.078 ± 0.008* (78.78)0.077 ± 0.006* (77.77)0.075 ± 0.001* (75.75)0.070 ± 0.003* (70.70)0.073 ± 0.006+ (75.25)
Star. + Hist. + Lim.0.074 ± 0.003* (74.74)0.071 ± 0.002* (71.71)0.072 ± 0.002* (72.72)0.068 ± 0.004* (68.68)0.073 ± 0.003+ (75.25)

Each value is mean ± SE of six replicates. Values in parentheses are percent change with control taken as 100%.
Concentration (w/v) has been expressed as final concentration in aquarium water. *Significant (P < 0.05) when t-test was applied in between treated and control groups and +in between 60% of 96 h LC50 and withdrawal group. Glu.: glucose; Star.: starch; Meth.: methionine; Hist.: histidine; Eug.: eugenol; Feb.: ferulic acid; Umb.: umbelliferone; Lim.: limonene.