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ISRN Biochemistry
Volume 2013 (2013), Article ID 198065, 5 pages
http://dx.doi.org/10.1155/2013/198065
Research Article

Biochemical Studies on Methylglyoxal-Mediated Glycated Histones: Implications for Presence of Serum Antibodies against the Glycated Histones in Patients with Type 1 Diabetes Mellitus

1Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India
2Department of Biochemistry, Universal College of Medical Sciences, Paklihawa Campus, Bhairawaha, Nepal

Received 17 June 2013; Accepted 17 July 2013

Academic Editors: K.-i. Isobe and J. Neira

Copyright © 2013 Nadeem A. Ansari and Debabrata Dash. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Reactive carbonyl species (RCS) mainly reacts with lysine and arginine residues of proteins to form advanced glycation end products (AGEs). Histone was glycoxidated with glyoxal and methylglyoxal. It was characterized by polyacrylamide gel electrophoresis and quenching studies involving penicillamine and aminoguanidine as carbonyl scavengers. Further characterization of histone modified with methylglyoxal was done by UV, fluorescence, and IR spectrophotometry. Spectral analysis of the protein clearly demonstrates structural perturbation in the histone by methylglyoxal. Methylglyoxal-induces cross-linking in the protein leading to aggregation. Role of methylglyoxal mediated glycoxidation of histone in type 1 diabetes was also undertaken. Antibodies were detected against glycoxidated histone in sera of type 1 diabetes patients by solid-phase enzyme immunoassay. The findings indicate that as a result of structural perturbation in histone by methylglyoxal, the modified histone may be involved in production of serum antibodies in the diabetes patients.