Table 1: UV and fluorescence data of native and methylglyoxal modified histones under identical experimental conditions.

Native histoneMethylglyoxal-modified histoneModification

UV absorbance at 320 nma0.220.3741.2%
UV absorbance at 320 nmb0.220.7676.2%
Fluorescence intensitya
(Ex. 320 nm)
222
(Em. 419 nm)
335
(Em. 413 nm)
33.7%
Fluorescence intensityb
(Ex. 320 nm)
222
(Em. 419 nm)
1220
(Em. 440 nm)
81.7%

a and b represent 1 and 2 mM concentrations, respectively, of the histone modifier.