Table 1: Summary of the purification of the nicking endonuclease from Streptomyces.

StepVolume (mL)Protein concentration (mg/mL)Total protein (mg)Specific activity (U/mg)Total units (U)Fold purification (x)Recovery (%)

Cell-free extract5711.66618.625,7001.0100
0–60% NH3SO42018.537010.84,0001.2570
DEAE cellulose35.516.554.59006.016
Phenyl-Sepharose10.20.210002001162