631875.fig.004
Figure 4: Calibration of chromatographic system. The oligonucleotides injected are 5′-T21-3′ (MW: 6326.2 g·mol−1), 5′-T24-3′ (MW: 7238.7 g·mol−1), 5′-ACC CCC TGC ATC TGC ATG CCC CCT CCC ACC CCC T-3′ (MW: 10,057.5 g·mol−1), 5′-ACC CCC TGC ATC TTT TTG CCC CCT CCC ACC CCC T-3′ (MW: 10,076.5 g·mol−1), and the Watson-Crick duplex formed by 5′-ACC CCC TGC ATC TGC ATG CCC CCT CCC ACC CCC T-3′ and 5′-AGG GGG TGG GAG GGG GCA TGC AGA TGC AGG GGG T-3′ (MW: 20,872.7 g·mol−1). All of these sequences form lineal structures at pH 7.1 and 20°C.