910308.fig.004a
(a) Catha edulis Forsk
910308.fig.004b
(b) Artemisia annua
910308.fig.004c
(c) Rumex abyssinicus
Figure 4: Exposure of Trypanosoma brucei cell to (a) Catha edulis Forsk (CEF), (b) Artemisia annua (AMR), and (c) Rumex abyssinicus (RMA) extracts. Cells ( cells per well) were seeded in 24 well plates in a volume of 1 mL per well, and 100 μL of extracts was inoculated in each well except in the controls where 100 μL of media is inoculated instead of the extracts. The cells were incubated at 37°C containing 5% CO2 in a 100% humidified environment for 24 hrs. Cell counts were made at 0, 1, 3, 6, and 24 hrs of incubation.