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ISRN Cell Biology
Volume 2012 (2012), Article ID 568104, 14 pages
http://dx.doi.org/10.5402/2012/568104
Research Article

Co-localization of the PDGF β-Receptor and Actin during PDGF Stimulation in Mouse Fibroblasts

Cell Biology, Department of Biology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

Received 23 October 2011; Accepted 13 November 2011

Academic Editors: G. Castoria, W. K. Song, and P. Storz

Copyright © 2012 Maarten J. A. Moes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The subcellular localization of the PDGF β-receptor was investigated in relation with PDGF-induced actin and membrane dynamics in mouse C3H10T1/2 fibroblasts. Serum-starved cells exhibit a nonhomogenous distribution of PDGF β-receptors. However, the observed pattern does not resemble the localization of PDGF-induced actin structures. Interestingly, the PDGF β-receptor showed a changed subcellular distribution in relation to the formation of PDGF-BB-induced actin structures. Upon PDGF exposure, PDGF β-receptors were found to accumulate in dorsal circular ruffles. The presence of both macropinosomes and clathrin in the induced circular ruffles suggests that the accumulation of PDGF β-receptors in circular ruffles results in the efficient internalization of PDGF β-receptors.