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Figure 3: Mitochondrial translocation of Parkin. HeLa cells expressing GFP-tagged Parkin (green) were treated with (+) or without (–) 10 μM CCCP for 3 hrs to disrupt the mitochondrial membrane potential (Δψm). Parkin was moved to mitochondria upon the reduction of Δψm (stained with mitochondrial outer membrane protein Tom20 (red), counterstained with DAPI (blue) for the nucleus). Scale bar = 10 μm. Original data were provided by Shiba-Fukushima et al.