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ISRN Chemical Engineering
Volume 2012 (2012), Article ID 601458, 5 pages
http://dx.doi.org/10.5402/2012/601458
Research Article

Determination of Oxygen Solubility in Liquid Media

1Chemical and Food Engineering Department, Federal University of Santa Catarina (UFSC), P.O. Box 476, 88040-900 Florianópolis, SC, Brazil
2Microbiology, Immunology and Parasitology Department, Federal University of Santa Catarina (UFSC), P.O. Box 476, 88040-900 Florianópolis, SC, Brazil

Received 23 February 2012; Accepted 26 April 2012

Academic Editors: G. Bayramoglu and A. Brucato

Copyright © 2012 Francielo Vendruscolo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present work aimed at determining the oxygen saturation in culture medium used in the production of pigments by Monascus ruber CCT 3802. This estimation allows the correction and the minimization of errors on the specific oxygen uptake rates determination because the conversion of oxygen partial pressure to oxygen concentration requires accurate information on oxygen solubility in experimental incubation media. By adding hydrogen peroxide and then transforming into water and oxygen using catalase, it was possible to determinate the saturation concentration of 7.677 and 6.772 mgO2 L āˆ’ 1 in distilled water and in growth medium, respectively. The determination of these parameters makes possible the minimization of errors on the specific oxygen uptake rates determination, once many studies consider the saturation concentration in distilled water.