Table 2: Magnitude of the kinetic constants in (13) to (16) for a few buffers, according to reference [44].

BufferpH (mM) (M−1 s−1) (mM)

Triethanolamine 8.4 7.8 1.2
HEPES* 8.2 7.5 3.5
4-methylimidazole 8.4 7.8 1.3
1-methylimidazole 8.0 7.2 2.4

*HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.