Table 1: Irrigation protocol.

GroupNo. of teethIrrigation during
cleaning and shaping
Final rinseImmersion

Positive control6Distilled waterdH2OdH2O
Negative control6Distilled waterdH2O and autoclavedH2O
A61.5% NaOCl, 1 mL after each file1.5% NaOCl, 5 mL1.5% NaOCl, 5 min
B63% NaOCl, 1 mL after each file3% NaOCl, 5 mL3% NaOCl, 5 min
C61.5% NaOCl, 1 mL after each fileMTAD, 5 mLMTAD, 5 min
D61.5% NaOCl, 1 mL after each file and EDTA 17%, 1 min1.5% NaOCl, 5 mL1.5% NaOCl, 5 min
E63% NaOCl, 1 mL after each file and EDTA 17%, 1 min3% NaOCl, 5 mL3% NaOCl, 5 min