834359.fig.002a
(a)
834359.fig.002b
(b)
834359.fig.002c
(c)
Figure 2: Evidence to suggest that the hybridization signal revealed by in situ hybridization (ISH) is over islet cell nuclei. (a) shows the distribution pattern and size (yellow bar = 10 μM) of receptor-negative islet cell nuclei in adult mouse pancreas as revealed by in situ ligand binding. (b) shows the distribution pattern and size (blue bar = 10 μM) of islet cell nuclei in adult mouse pancreas as revealed by hematoxylin-eosin staining. Manual counting revealed 262 hematoxylin-positive nuclei in this tissue section amounting to a density of 0.93 nuclei/arbitrary unit of islet area. (c) shows the distribution pattern and size (black bar = 10 μM) of presumptive islet cell nuclei in adult mouse pancreas as revealed by ISH. All focal points of hybridization signal over the islets are similar in distribution pattern and size to the nuclei in (b). Manual counting revealed 285 presumptive nuclei in this tissue section amounting to a density of 0.92 nuclei/arbitrary unit of islet area.