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ISRN Gastroenterology
Volume 2012 (2012), Article ID 762920, 7 pages
http://dx.doi.org/10.5402/2012/762920
Research Article

Effect of Antioxidant Treatment on Fibrogenesis in Rats with Carbon Tetrachloride-Induced Cirrhosis

1Post-Graduation Medical Sciences Program, Medical School, Federal University of Rio Grande do Sul (UFRGS), 90035-903 Porto Alegre, RS, Brazil
2Laboratory of Molecular Biology of Autoimmune and Infectious Disease, Hospital de Clínicas de Porto Alegre (HCPA), 90035-903 Porto Alegre, RS, Brazil
3Department of Physiology, Universidade Federal do Rio Grande do Sul (UFRGS), 90040-060 Porto Alegre, RS, Brazil
4Physiotherapy Course, Universidade Católica de Pelotas (UCPEL), 96010-000 Pelotas, RS, Brazil
5Post-Graduation Physiology, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, Brazil
6Academic Course of Medicine, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil
7Post-Graduation Medical Sciences Program, Instituto de Cardiologia do Rio Grande do Sul, 91045-140 Porto Alegre, RS, Brazil
8Laboratory of Molecular Biology of Autoimmune and Infectious Diseases, HCPA, 90035-903 Porto Alegre, RS, Brazil
9Laboratory of Experimental Gastroenterology and Hepatology—Federal University of Rio Grande do Sul (UFRGS), 92425-900 Canoas, RS, Brazil
10Laboratory of Oxidative Stress and Antioxidants—Lutheran University of Brazil (ULBRA), 92425-900 Canoas, RS, Brazil

Received 5 December 2011; Accepted 28 December 2011

Academic Editors: G.-T. Huang and G. D. Mazzolini

Copyright © 2012 Silvia Bona et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. This study aimed to assess the antioxidant activity of quercetin (Q) in an experimental model of cirrhosis induced by CCl4 inhalation. Materials and Methods. We used 25 male Wistar rats (250 g) that were divided into 3 groups: control (CO), CCl4, and C C l 4 + Q . The rats were subjected to CCl4 inhalation (2x/week) for 16 weeks, and they received phenobarbital in their drinking water at a dose of 0.3 g/dL as a P450 enzyme inducer. Q (50 mg/Kg) was initiated intraperitoneally at 10 weeks of inhalation and lasted until the end of the experiment. Statistical analysis was by ANOVA Student Newman-Keuls ( m e a n ± S E M ), and differences were considered statistically significant when 𝑃 < 0 . 0 5 . Results. After treatment with quercetin, we observed an improvement in liver complications, decreased fibrosis, as analyzed by picrosirius for the quantification of collagen, and decreased levels of matrix metalloproteinase 2 (MMP-2) compared with the CCl4 group. It also reduced oxidative stress, as confirmed by the decrease of substances reacting to thiobarbituric acid (TBARS), the increased activity of antioxidant enzymes, and the reduced glutathione ratio and glutathione disulfide (GSH/GSSG). Conclusion. We suggest that the use of quercetin might be promising as an antioxidant therapy in liver fibrosis.