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ISRN Immunology
Volume 2012 (2012), Article ID 853123, 17 pages
http://dx.doi.org/10.5402/2012/853123
Review Article

Host Defence against Bacterial Biofilms: “Mission Impossible”?

Institute for Immunology, University of Heidelberg, 69120 Heidelberg, Germany

Received 11 September 2012; Accepted 4 October 2012

Academic Editors: C. Faveeuw, D. Lilic, P. Puccetti, and A. Taylor-Robinson

Copyright © 2012 Gertrud Maria Hänsch. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Explanation for the video clips: Isolated PMN, labelled with calcein (green) were placed on a S. aureus biofilm, stained red. PMN move across the biofilm. In their wake, areas depleted of biofilm appear (greyish colour). Some PMN turn yellow, indicative of biofilm uptake. Clip 1 shows a solid biofilm (S. aureus, 6 days grown inculture; clip 2 a fragmented biofilm). With the fragments, the "chase" of the PMN towards the biofilm and the phagocytosis of biofilm material can be observed rather nicely. On the upper left corner, the time is shown in minutes (the video clip was provided by Dr. Frank G√ľnther, Department of Microbiology, University of Heidelberg, Germany).

  1. Supplementary Video 1
  2. Supplementary Video 2