Figure 4: Effects of LPS injection. (a) Tumor measurements on different timepoints. (b) Total lymph node cells in mice with B16F10 injection (○), Injection of LPS at the same time of B16F10 injection (▾); Injection of LPS 18 hours before B16F10 injection (∆); Intratumoral LPS five days after B16F10 injection (□) and PBS injection (*). Values of were considered statistically significant. Data representative of three different experiments, mice per group. (c) TLR4 expression on B16F10 cells by RT PCR. Agarose gel eletrophoresis analysis of PCR products Lane 1 B16F10, cDNA Lane 2 Negative control, Lane 3 Ladder 100 bp (Invitrogen).