Table 1: Nucleotide sequence of primers used in genomic walking. Overhanging primers (OHP) with KpnI, PstI, and SacI overhang sequence indicated in italic bold adaptor primer (AP), nested primer (NP), and Adh gene specific primer (GSP4).

Primer name Sequence

OHP KpnI5′-GAATTCGAGCTCGCCCGGGATCCTCTAGAGTAC -3′
OHP PstI5′-GAATTCGAGCTCGCCCGGGATCCTCTAGATGCA -3′
OHP SacI5′-GAATTCGAGCTCGCCCGGGATCCTCTAGAAGCT -3′
AP5′-GAATTCGAGCTCGCCCGGGAT-3′
NP5′-GCTCGCCCGGGATCCTCTAGA-3′
GSP45′-GCCCTTAGCTTCCCAGAAGT-3′