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ISRN Obstetrics and Gynecology
Volume 2012 (2012), Article ID 514146, 5 pages
http://dx.doi.org/10.5402/2012/514146
Clinical Study

HPV Type Distribution and Cervical Cytology among HIV-Positive Tanzanian and South African Women

1Erasmus MC, University Medical Center, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands
2Leiden Cytology and Pathology Laboratory, P.O. Box 16084, 2301 GB Leiden, The Netherlands
3Lawson Health Research Institute, Canadian Research and Development Centre for Probiotics and Departments of Microbiology, Immunology and Surgery, University of Western Ontario, London, Canada N6A 4V2
4Department of Epidemiology, University of California Los Angeles, Los Angeles, CA 90095, USA
5Ndlovu Care Group, Elandsdoorn, P.O. Box 15008, Groblersdal 0470, South Africa
6Bontekoe Research, Rooseveltstraat 4-d, 2321 BM Leiden, The Netherlands
7DDL Diagnostic Laboratory, Visseringlaan 25, 2288 ER Rijswijk, The Netherlands

Received 26 February 2012; Accepted 6 May 2012

Academic Editors: K. Chan, M. KΓΌhnert, E. Porcu, and L. C. Zeferino

Copyright © 2012 Joke A. M. Dols et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. There are limited data on high-risk human papillomavirus (hr-HPV) genotypes among HIV-positive women in Africa, and little is known about their relationship with cervical cytology in these populations. Methods. We conducted a cross-sectional study among 194 HIV-positive women (143 from Tanzania, and 51 from South Africa) to evaluate HPV genotypes among HIV-positive women with normal and abnormal cytology. Cervical samples were genotyped for HPV types, and slides were evaluated for atypical squamous cell changes according to the Bethesda classification system. Results. Prevalence of high grade squamous intraepithelial dysplasia (HSIL) was 9%. Overall, more than half (56%) of women were infected with an hr-HPV type; 94% of women with HSIL ( 𝑛 = 1 6 ), 90% of women with LSIL ( 𝑛 = 3 5 ), and 42% of women within normal limits (WNL) ( 𝑛 = 5 8 ) tested positive for hr-HPV. Overall, the most prevalent hr-HPV subtypes were HPV16 (26%) and HPV52 (30%). Regional differences in the prevalence of HPV18 and HPV35 were found. Conclusion. Regional differences in HPV genotypes among African women warrant the need to consider different monitoring programmes for cervical preneoplasia. HPV-based screening tests for cervical preneoplasia would be highly inefficient unless coupled with cytology screening of the HPV-positive sample, especially in HIV-positive women.