492578.fig.001a
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492578.fig.001b
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492578.fig.001c
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492578.fig.001d
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Figure 1: Example of PIK3CA E545 K mutation detection from a CT-guided biopsy sample (Case 2).(a) Section from a CT-guided biopsy sample stained with hematoxylin and eosin. The bar shows a distance of 50 μm. (b) Closeup of the same section showing the presence of breast cancer cells (indicated by red arrows). (c) High-resolution melting detection of a heterozygous E545 K mutation in DNA isolated from the sample (blue line, T47D DNA; red line, MCF7 DNA; green line, sample DNA). (d) Confirmation of mutation by Sanger sequencing. The red arrrow indicates the presence of a heterozygous mutation changing the codon GAG to AAG.