Research Article

Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages: Role in the Potentiation of Cellular Responses to LPS and LTA

Figure 1

Human myeloid ML-1 cell treatment with TPA is associated with the induction macrophages specific protein CD14 expression at both mRNA and protein levels. ML-1 cells were incubated in the presence or in the absence of 5 nM TPA for the indicated times. (a) CD14 mRNA levels were determined by examination of microarray analysis data (see Section 2). (b) The levels of CD14 protein expression on TPA-treated ML-1 cells were determined by FACS-Scan analysis using Alexa-Fluor 488 labeled anti-human CD14 antibodies. Negative controls were stained with isotype-matched- (Alexa-Fluor 488) conjugated IgG, and compensation was adjusted using the single-stained cell samples. The fluorescence intensities were determined using Cellquest software (Becton Dickinson, Bedford, MA). Data were analyzed by ModFILT statistical software. The figure depicts representative results from one of three replicate experiments.
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(a)
641246.fig.001b
(b)