768190.fig.004a
(a)
768190.fig.004b
(b)
Figure 4: CAI expression in cell lines. (a) Immunofluorescence detection of CAI in normal prostate epithelial cells and prostate cancer cells. The cells were fixed for 1 h, permeabilized, and stained with antibodies to detect CAI. Alexa Fluor 488- or Alexa Fluor 568-phalloidin was used to visualize normal prostate epithelial cells (upper panels) and prostate cancer cells of 22Rv1 (lower panels). (b) Western blot analysis of media conditioned by the normal prostate epithelial cells and prostate cancer cells probed using a CAI antibody. C3b-α was used as a loading control.