789152.fig.003a
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789152.fig.003b
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789152.fig.003c
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789152.fig.003d
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789152.fig.003e
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Figure 3: RMS cells show intact IFNRs and STAT1 phosphorylation in vitro. (a, b, and c) Flow cytometry data of IFNGR subunit expression in RMS and HT29 control cells lines; filled histograms represent expression levels using specific antibodies; open histograms represent isotype control stainings; (d) mutation analysis of STAT1 phosphorylation site and JAK binding motif in RMS cell lines and HT29 control cells; (e) western blot analysis of p STAT1 induction 24 h and 48 h after IFNγ treatment; β-actin serves as loading control.