Table 1: Sources, dilution, distribution, cut-offs point, and pretreatment used for revalidation.

AntibodyCloneSourceDilutionDistributionScoring SystemCut-offs PretreatmentPositive controlNegative control

Bcl-2124Dako-Cytomation1 : 100Cytoplasm% of positive cells>10% (positive) Antigen retrieval microwaveNormal breast aciniOmitting the antibody
BRCA1Ab-1 (MS110)Calbiochem1 : 150Nuclear% of positive cells<25% (negative)
Antigen retrieval microwaveMCF 7 cells Omitting the antibody
Ck5/6M7237Dako-Cytomation1 : 60Cytoplasm% of positive cells≥10% (positive) Antigen retrieval microwaveKnown case of CK56 BC Omitting the antibody
E-cadherinNCH-38Dako-Cytomation1 : 100Cytoplasm and membrane% of positive cells≥100 H score (positive)Antigen retrieval microwaveNormal gastric mucosaOmitting the antibody
EGFR31G7Novocastra1 : 30Membrane% of positive cells≥10% (positive)Not requiredMyoepithelial cells of normal duct in normal mammary glandOmitting the antibody
erbB2PolyclonalDako-Cytomation1 : 100MembraneTable 2.1Table 2.1Not requiredKnown case of erbB2 strong BC expressionOmitting the antibody
ER1D5Dako-Cytomation1 : 200Nuclear% of positive cells≥0 (positive) Antigen retrieval microwaveNormal breast aciniOmitting the antibody
Ki-67MIB1Dako-Cytomation1 : 25Nuclear% of positive cells<10% (low)
Antigen retrieval microwaveHuman tonsil tissueOmitting the antibody
P-cadherinNCL-P-cad
Novocastra
1 : 200Cytoplasm% of positive cells≥5% (positive)Antigen retrieval microwaveKnown case of P-cadherin strong BC expressionOmitting the antibody
PgRPgRDako-Cytomation1 : 150Nuclear% of positive cells ≥0 (positive) Antigen retrieval microwaveNormal breast aciniOmitting the antibody
p21EA10Abcam1 : 25Nuclear% of positive cells≥10% (positive) Antigen retrieval microwaveNormal breast aciniOmitting the antibody
p53 DO7Novocastra1 : 50Nuclear% of positive cells>10% (negative)
Antigen retrieval microwave Normal breast aciniOmitting the antibody