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ISRN Otolaryngology
Volume 2013 (2013), Article ID 479482, 6 pages
http://dx.doi.org/10.1155/2013/479482
Research Article

Detection of Otosclerosis-Specific Measles Virus Receptor (Cd46) Protein Isoforms

1Bajcsy-Zsilinszky Hospital, Department of Otolaryngology, Budapest, Hungary
2University of Debrecen, Medical and Health Science Center, Department of Rheumatology, Debrecen, Hungary
3University of Debrecen, Medical and Health Science Center, Department of Otolaryngology and Head and Neck Surgery, Nagyerdei Krt. 98, Debrecen 4032, Hungary

Received 12 May 2013; Accepted 5 June 2013

Academic Editors: D. C. Alpini, K. Ishikawa, T. Just, M. Sone, and D. Thurnher

Copyright © 2013 Balázs Liktor et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Genetic predisposition of otosclerosis has long been suspected, but unclarified. Unique coexpression pattern of measles virus receptor (CD46) splicing isoforms in the human otic capsule is assumed, since otosclerosis is a measles virus-associated organ-specific disease. In order to identify CD46 involved in the pathogenesis of otosclerosis, we used representative groups of histologically diagnosed otosclerotic, nonotosclerotic, and normal stapes footplates . Consecutive histopathological examinations and CD46-specific Western blot analysis were performed. Normal and nonotosclerotic stapes footplates showed consistent expression of the conventional c, d, e, f, and l CD46 isoforms. In contrast, four novel isoforms (os1–4) translated as intact proteins were additionally detected in each otosclerotic specimen. The study herein presented provides evidence for the otosclerosis-associated expression pattern of CD46. This finding might explain the organ-specific, virus-associated and autoimmune-inflammatory pathogenesis of otosclerosis. Regarding our current knowledge, this is the first report that confirms the presence of four new disease-specific protein variants of CD46.