930729.fig.002a
(a)
930729.fig.002b
(b)
930729.fig.002c
(c)
Figure 2: Schematic representation of design for building of plasmids. Positions of primers used for verification of both insertion and orientation of insert are indicated in boxes. The sequences of primers used are as follows. IU: 5′-atccacgctgttttgacctc-3′, IL: 5′-atgctattgctttatttgta-3′, FBP10: 5′-tgcacatcagccaccatgga-3′, and GS12: 5′-aatttcaacgtggaaaaccct-3′.