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ISRN Pharmaceutics
Volume 2012 (2012), Article ID 216068, 5 pages
http://dx.doi.org/10.5402/2012/216068
Research Article

Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry

Department of Chemistry, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran

Received 23 October 2012; Accepted 7 November 2012

Academic Editors: J. Lee, L. Pezza, and C. Zacharis

Copyright © 2012 Mehdi Taghdiri and Arash Mohamadipour-taziyan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps. Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution. This attenuation was used to the microdetermination of dopamine by measurement of absorbance of the solid phase (Sephadex LH-20 gel) in a 1.0 mm cell at 625 nm. Dopamine could be determined in the concentration range of 0.4–1.6 μg mL−1 (10-mL Sample volume) with a relative standard deviation (RSD) of 0.03% ( ). The detection limit was obtained, 0.26 μg mL−1 (1.7 μM). The method was used for determination of dopamine in pharmaceutical injection sample and satisfactory result was obtained.