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ISRN Pharmacology
Volume 2012 (2012), Article ID 410902, 6 pages
http://dx.doi.org/10.5402/2012/410902
Research Article

Nephroprotective Effect of Ursolic Acid in a Murine Model of Gentamicin-Induced Renal Damage

1Department of Pharmacology, Kasturba Medical College, Manipal University, Mangalore 575001, Karnataka, India
2Department of Pathology, K S Hegde Medical Academy, Nitte University, Deralakatte, Mangalore 574160, India
3Department of Physiology, Father Muller Medical College, Mangalore 575002, India

Received 4 April 2012; Accepted 3 May 2012

Academic Editors: J. L. Cornish and M. A. Prendergast

Copyright © 2012 Preethi G. Pai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study evaluates the nephroprotective effects of ursolic acid in a murine model of gentamicin induced renal damage. Wistar albino rats of either sex, weighing 150–200 g were divided into 5 groups; normal saline, gentamicin 80 mg/kg, intraperitoneally for 8 days, ursolic acid at 2, 5, and 10 mg/kg, per oral for 8 days, ursolic acid administered 3 days prior and concurrently with gentamicin for 5 days. Blood urea, serum creatinine, uric acid and blood urea nitrogen analyses and microscopic examination of kidney were performed. Gentamicin treatment caused nephrotoxicity as evidenced by marked elevation in serum urea, serum uric acid, serum creatinine and blood urea nitrogen (162.33 ± 9.92 mg/dL, 3.13 ± 0.12 mg/dL, 6.85 ± 0.35 mg/dL and 75.86 ± 4.64 mg/dL; resp.) when compared to the saline treated groups. Co-administration of ursolic acid with gentamicin decreased the rise in these parameters in a dose dependent manner. Histopathological analysis revealed epithelial loss with intense granular degeneration in gentamicin treated rats, whereas ursolic acid mitigated the severity of gentamicin-induced renal damage. To conclude, our data suggest that ursolic acid exhibits renoprotective effect in gentamicin induced renal damage and further studies on its mechanis of action are warranted.