Table 1: Oligonucleotides and cycling conditions for PCR amplification of p53, bcl-2, Bax, caspase 9, caspase 3, and 18S.

GenePrimer sequencesATCSize (bp)

p53Forward—CTG CCC ACC ACA GCG ACA GG59°C35471
Reverse—AGG AGC CAG GCC GTC ACC AT
Bcl-2Forward—GGG CTA CGA GTC GGA TAC53°C3564
Reverse—AGG CTG GAA GGA GAA GAT G
BaxForward—CGC GTG GTT GCC CTC TTC TAC TTT 59°C35124
Reverse—CAA GCA GCC GCT CAC GGA GGA
Caspase 9Forward—TGC AGG GTA CGC CTT GTG CG61°C35130
Reverse—CCT GAT CCC GCC GAG ACC CA
Caspase 3Forward—AGG CCT GCC GAG GTA CAG AGC61°C35255
Reverse—CCG TGG CCA CCT TCC GCT TA
18sForward—AAG ACG AAC CAG AGC GAA AG56°C25149
Reverse—GGC GGG TCA TGG GAA TAA

AT: annealing temperature; C: number of cycles during exponential phase of amplification.