Table 1: Bacterial strains and plasmids used in this study.

Strains and plasmidsDescriptionSource

Lactobacillus brevis DT24Probiotic vaginal isolateIn this study
Escherichia coli NCTC 50133Contains pColE2-P9, produces colicin E2[50]
Escherichia coli DH5 Transformation hostMTCC
Escherichia coli BL21 D3Expression HostMTCC
Escherichia coli Uropathogenic strainMTCC

PlasmidsDescriptionSource

pSLP111.3Cmr, E. coli/LAB shuttle vector
SlpA signal peptide
Prof. Jos Seeger
Lactrys, The Netherlands
pColE2-P9Stpr, vector for colicin E2E. coli Genetic Stock Centre, Yale University
pSL-ColE2Cmr, SlpA, and ColE2In this study