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ISRN Veterinary Science
Volume 2011 (2011), Article ID 183412, 6 pages
Research Article

Single Layer Centrifugation Can Be Scaled-Up Further to Process up to 150 mL Semen

1Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden
2Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
3Quality Genetics, 24292 Hörby, Sweden

Received 6 October 2011; Accepted 20 November 2011

Academic Editor: K.-P. Brüssow

Copyright © 2011 J. M. Morrell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Single-Layer centrifugation has been used to improve the quality of sperm samples in several species. However, where stallion or boar semen is to be used for AI, larger volumes of semen have to be processed than for other species, thus limiting the effectiveness of the original technique. The objective of the present study was to scale up the SLC method for both stallion and boar semen. Stallion semen could be processed in 100 mL glass tubes without a loss of sperm quality, and similarly, boar semen could be processed in 200 mL and 500 mL tubes without losing sperm quality. The results of these preliminary studies are encouraging, and larger trials are underway to evaluate using these methods in the field.